Physiological and biochemical changes in Moroccan barley (Hordeum vulgare L.) cultivars submitted to drought stress

Barley (Hordeum vulgare L.) is the second most consumed and cultivated cereal by the Moroccan population. However, it is predicted that frequent drought periods, caused by climate change, can cause problems in plant growth. Thus, the selection of drought-tolerant barley cultivars is essential to ensure the security of barley's needs. We aimed to screen drought stress tolerance in Moroccan barley cultivars. We tested the drought tolerance of nine Moroccan barley cultivars ('Adrar', 'Amalou', 'Amira', 'Firdaws', 'Laanaceur', 'Massine', 'Oussama', 'Taffa', and 'Tamellalt') based on physiological and biochemical parameters. Drought stress was applied by maintaining field capacity at 40% (90% for the control), and plants were randomly arranged in a greenhouse at 25 degrees C under natural light conditions. Drought stress decreased relative water content (RWC), shoot dry weight (SDW), and chlorophyll content (SPAD index), but significantly increased electrolyte leakage, hydrogen peroxide, malondialdehyde (MDA), water-soluble carbohydrates, and soluble protein contents, as well as catalase (CAT) and ascorbate peroxidase (APX) activities. High levels of SDW, RWC, CAT, and APX activities were recorded in 'Firdaws', 'Laanaceur', 'Massine', 'Taffa', and 'Oussama', which can be interpreted by high drought tolerance. On the other hand, 'Adrar', 'Amalou', 'Amira', and 'Tamellalt' showed higher values of MDA and H2O2 content, which can be linked with drought sensitivity. Physiological and biochemical parameter changes are discussed in terms of barley's tolerance to drought. Tolerant cultivars could be a good background for barley breeding in areas known for the alternative of long dry spells.