A Label-Free Deoxyribozyme Sensor for m6A Demethylase Activity Detection

被引：1
|
作者：
Wang, Xiuyuan [1 ]
Yu, Shusheng [1 ]
He, Yuqiu [1 ]
Wang, Fuan [1 ]
Liu, Xiaoqing [1 ]
机构：
[1] Wuhan Univ, Coll Chem & Mol Sci, Wuhan 430072, Hubei, Peoples R China
来源：
CHINESE JOURNAL OF CHEMISTRY | 2024年 / 42卷 / 09期
基金：
中国国家自然科学基金;
关键词：
DNAzyme; Analytical methods; Biosensing; m(6)A demethylation; Fluorescence; FAMILIAL MEDITERRANEAN FEVER; SENSITIVE DETECTION; DNA DEMETHYLATION; TURKISH PATIENTS; G-QUADRUPLEX; GENE; CHILDREN; METHYLTRANSFERASE; MANIFESTATIONS; DIAGNOSIS;
D O I：
10.1002/cjoc.202300633
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
A label-free deoxyribozyme (DNAzyme) biosensor for m(6)A demethylase activity detection is developed. When demethylase FTO (Fat mass and obesity-associated protein), an important m(6)A demethylase of ALKBH demethylase family is present, the m(6)A group is specifically demethylated, and the cleavage activity of DNAzyme is restored. This allows the cleavage product that contains G-quadruplex sequence to bind to thioflavin T (ThT) and generate fluorescence signals. The biosensor shows high specificity and sensitivity, and fast reaction speed. Our study demonstrates a new design of allosteric DNAzyme for sensing. This method represents the first label-free nucleic acid biosensor for FTO assay, providing a feasible route towards inhibitors screening. [GRAPHICS]
引用
收藏
页码：951 / 956
页数：6
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