Development of a Real-Time PCR Assay for the Detection of the Phlebovirus Fermo Virus

被引：5

作者：

Calzolari, Mattia ^{[1
]}

Russo, Simone ^{[1
]}

Marzani, Katia ^{[1
]}

Dalmonte, Gastone ^{[1
]}

Ricchi, Matteo ^{[1
]}

Bonilauri, Paolo ^{[1
]}

机构：

[1] Ist Zooprofilatt Sperimentale Lombardia & Emilia R, I-25124 Brescia, Italy

来源：

VIRUSES-BASEL | 2023年 / 15卷 / 10期

关键词：

Fermo virus; sand fly; real-time PCR; digital PCR; IDENTIFICATION; TOSCANA;

D O I：

10.3390/v15102082

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Fermo virus is a Phlebovirus that is increasingly reported in sand flies from northern Italy. The natural cycle is not fully understood, but the virus has been detected by direct methods only in sand flies. Although there is serological evidence that it can infect vertebrates, the virus has not been directly detected in animals or humans. Here, we have developed and reported a specific real-time PCR for Fermo virus. The availability of the described method will be useful to characterize the epidemiology of the FERV, ensuring, compared to previously available protocols, a more sensitive detection in insects and the possible detection in vertebrates to evaluate the presence of reservoirs and the pathogenic potential of the virus in humans or animals.

引用

页数：8

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