Phytoplankton community composition in relation to environmental variability in the Urdaibai estuary (SE Bay of Biscay): Microscopy and eDNA metabarcoding

被引：7

作者：

Bilbao, Jone ^{[1
,2
]}

Pavloudi, Christina ^{[3
,4
]}

Blanco-Rayon, Esther ^{[2
]}

Franco, Javier ^{[5
]}

Madariaga, Iosu ^{[1
]}

Seoane, Sergio ^{[1
,2
]}

机构：

[1] Univ Basque Country UPV EHU, Fac Sci & Technol, Dept Plant Biol & Ecol, Leioa, Spain

[2] Univ Basque Country, Res Ctr Expt Marine Biol & Biotechnol, Plentzia Marine Stn, PiE UPV EHU, Plentzia, Spain

[3] Hellen Ctr Marine Res HCMR, Inst Marine Biol Biotechnol & Aquaculture IMBBC, Iraklion, Crete, Greece

[4] George Washington Univ, Dept Biol Sci, Washington, DC 20052 USA

[5] AZTI, Marine Res Div, Pasaia, Spain

来源：

MARINE ENVIRONMENTAL RESEARCH | 2023年 / 191卷

关键词：

Microalgae; Taxonomy; Environmental gradients; Eutrophic waters; Estuaries; HARMFUL ALGAL BLOOMS; TEMPERATE ESTUARY; MINUTOCELLUS-POLYMORPHUS; INTERANNUAL VARIABILITY; TEMPORAL VARIABILITY; ASSEMBLAGE STRUCTURE; SUCCESSION PATTERNS; INTERTIDAL MUDFLAT; SOUTHEASTERN BAY; COASTAL WATERS;

D O I：

10.1016/j.marenvres.2023.106175

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Phytoplankton monitoring is essential for the global understanding of aquatic ecosystems. The present research studies the phytoplankton community of the Urdaibai estuary, combining microscopy and eDNA metabarcoding for the first time in the area. The main aims were to describe the phytoplankton community composition in relation to the environmental conditions of the estuary, and to compare the two methods used. Diatoms Minutocellus polymorphus and Chaetoceros tenuissimus dominated the outer estuary, being replaced by Teleaulax acuta (cryptophyte), Kryptoperidinium foliaceum (dinoflagellate) and Cyclotella spp. (diatom) towards the inner area. This change was mainly prompted by salinity and nutrients. Metabarcoding revealed the presence of 223 species that were not observed by microscopy in previous studies in the estuary. However, several characteristic species (e.g., K. foliaceum) were only detected with microscopy. Additionally, microscopy covered the limitations of eDNA metabarcoding concerning quantification. Thus, to give a full insight, a combination of techniques is recommended.

引用

页数：18

共 148 条

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