In Vitro and In Vivo Evaluation of Epidermal Growth Factor (EGF) Loaded Alginate-Hyaluronic Acid (AlgHA) Microbeads System for Wound Healing

被引：2
作者：
Ali, Maqsood [1 ]
Kwak, Si Hyun [2 ]
Byeon, Je Yeon [2 ]
Choi, Hwan Jun [2 ]
机构：
[1] Soonchunhyang Univ, Coll Med, Dept Regenerat Med, Cheonan 31538, South Korea
[2] Soonchunhyang Univ, Coll Med, Dept Plast & Reconstruct Surg, Cheonan 31538, South Korea
来源：
JOURNAL OF FUNCTIONAL BIOMATERIALS | 2023年 / 14卷 / 08期
基金：
新加坡国家研究基金会;
关键词：
alginate; hyaluronic acid; heparin; epidermal growth factor; FACTOR RECEPTOR; OXIDIZED ALGINATE; SUSTAINED-RELEASE; HEPARIN; HYDROGEL; EXPRESSION; DELIVERY; DEGRADATION; BINDING; CELLS;
D O I：
10.3390/jfb14080403
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
The management of skin injuries is one of the most common concerns in medical facilities. Different types of biomaterials with effective wound-healing characteristics have been studied previously. In this study, we used alginate (Alg) and hyaluronic acid (HA) composite (80:20) beads for the sustained release of epidermal growth factor (EGF) delivery. Heparin crosslinked AlgHA beads showed significant loading and entrapment of EGF. Encapsulated beads demonstrated biocompatibility with rat L929 cells and significant migration at the concentration of AlgHAEGF100 and AlgHAEGF150 within 24 h. Both groups significantly improved the expression of Fetal Liver Kinase 1 (FLK-1) along with the Intercellular Adhesion Molecule-1 (ICAM-1) protein in rat bone Mesenchymal stem cells (rbMSCs). In vivo assessment exhibited significant epithelialization and wound closure gaps within 2 weeks. Immunohistochemistry shows markedly significant levels of ICAM-1, FLK-1, and fibronectin (FN) in the AlgHAEGF100 and AlgHAEGF150 groups. Hence, we conclude that the EGF-loaded alginate-hyaluronic acid (AlgHA) bead system can be used to promote wound healing.
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页数：17
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