Detection of non-ribosomal and polyketide biosynthetic genes in bacteria from green mud crab Scylla serrata gut microbiome and their antagonistic activities

被引：2

作者：

Banu, Shabreen ^{[1
]}

Alva, Shivakiran ^{[1
]}

Prabhu, Prathiksha J. ^{[1
]}

Krishnan, Sreedharan ^{[2
]}

Mani, Madhu K. ^{[1
]}

机构：

[1] Nitte, Nitte Univ Ctr Sci Educ & Res NUCSER, Paneer Campus,, Mangalore 575018, Karnataka, India

[2] ICAR Cent Inst Fisheries Educ, Via Anwal, Rohtak 124411, Haryana, India

来源：

FISH AND SHELLFISH IMMUNOLOGY REPORTS | 2023年 / 5卷

关键词：

Biosynthetic genes; Mud crab; Gut bacteria; PCR; Antimicrobial activity; MARINE; EXOPOLYSACCHARIDES; ANTIBIOTICS; METABOLITES; DIVERSITY; DISCOVERY; SEQUENCES;

D O I：

10.1016/j.fsirep.2023.100104

中图分类号：

S9 [水产、渔业];

学科分类号：

0908 ;

摘要：

Multi-modular enzyme complexes known as non-ribosomal peptide synthetases (NRPSs) and polyketide syn-thetases (PKSs) have been widely reported in bacteria that produce secondary bioactive metabolites such as non -ribosomal peptides (NRPs) and polyketides (PKs), respectively. These NRPS/PKS pathways contribute to syn-thesizing several antibiotics, such as vancomycin, rifamycin, and bleomycin, which are vital in human medicine. The present study aimed to isolate gut-associated bacteria from mud crab Scylla serrata, and detect NRPS and PKS gene clusters associated with it. This study included 36 bacterial isolates from five mud crab gut samples. Biosynthetic gene clusters (NRPS and PKS), were detected by PCR using degenerative primers specific to these genes. Three isolates (FKP2-4, FKP4-1, and FKP2-16) were positive for NRPS and two for PKS (FKP2-4 and FKP4-1) genes. The isolates were subjected to 16S rRNA gene amplification and sequenced. In silico analysis of the sequences using the Basic Local Alignment Search Tool (BLAST) identified the isolates FKP2-4, FKP4-1, and FKP2-16 as Acinetobacter variabilis, Vagococcus fluvialis, and Staphylococcus arlettae, respectively, after comparing with the existing sequences available in the National Center for Biotechnology Information (NCBI) database. Compared to the control, it was observed that these isolates exhibited intriguing antagonistic activities against Escherichia coli and Staphylococcus aureus. However, these isolates failed to show significant activity against Candida albicans. Exopolysaccharide production by the isolated organisms was tested using Zobell marine agar (ZMA) with 5% sucrose, but none of the colonies were mucoid or slimy.

引用

页数：6