Rab7/Retromer-based endolysosomal trafficking is essential for proper host invasion in rice blast

被引：15

作者：

Chen, Xin ^{[1
,2
]}

Selvaraj, Poonguzhali ^{[3
]}

Lin, Lili ^{[1
,4
]}

Fang, Wenqin ^{[1
,2
]}

Wu, Congxian ^{[1
,5
]}

Yang, Piao ^{[1
,2
]}

Zhang, Jin ^{[1
,2
]}

Abubakar, Yakubu Saddeeq ^{[1
,2
]}

Yang, Fan ^{[3
]}

Lu, Guodong ^{[1
,2
]}

Liu, Wende ^{[4
]}

Wang, Zonghua ^{[1
,6
]}

Naqvi, Naweed I. ^{[3
]}

Zheng, Wenhui ^{[1
,2
]}

机构：

[1] Fujian Agr & Forestry Univ, Coll Plant Protect, State Key Lab Ecol Pest Control Fujian & Taiwan Cr, Fuzhou 350002, Peoples R China

[2] Fujian Agr & Forestry Univ, Coll Plant Protect, Key Lab Biopesticide & Chem Biol, Minist Educ, Fuzhou 350002, Peoples R China

[3] Natl Univ Singapore, Dept Biol Sci, Temasek Life Sci Lab, Singapore 117604, Singapore

[4] Chinese Acad Agr Sci, Inst Plant Protect, State Key Lab Biol Plant Dis & Insect Pests, Beijing 100193, Peoples R China

[5] Fujian Med Univ, Publ Technol Serv Ctr, Fuzhou 350122, Peoples R China

[6] Minjiang Univ, Inst Oceanog, Fuzhou 350108, Peoples R China

来源：

NEW PHYTOLOGIST | 2023年 / 239卷 / 04期

基金：

新加坡国家研究基金会; 中国国家自然科学基金;

关键词：

effector secretion; endolysosomal trafficking; Magnaporthe oryzae; Rab GTPase MoRab7; retromer; SNARE; MAGNAPORTHE-ORYZAE; GENE-EXPRESSION; TRIGGERED IMMUNITY; AUTOPHAGY; RETROMER; SECRETION; PATHOGENICITY; INFECTION; EFFECTORS; PATHWAYS;

D O I：

10.1111/nph.19050

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

<bold> Secretion is a fundamental process that plant pathogens utilize to deliver effectors into the host to downregulate immunity and promote infection</bold>. <bold> Here</bold>, we uncover a fascinating membrane trafficking and delivery route that originates from vacuolar membranes in Magnaporthe oryzae and conduits to the host interface and plasma membrane. <bold> To perform such secretory</bold>/trafficking function, MoRab7 first recruits the retromer complex to the vacuolar membrane, enabling recognition of a family of SNARE proteins, including MoSnc1. Live-cell imaging confirmed a highly dynamic vesicular trafficking of the retromer complex component(s) and MoSnc1 toward and across the host interface or plasma mem-brane, and subsequent fusion with target membranes. Interestingly, disruption of the MoRab7/Retromer/MoSnc1-based endolysosomal cascade affects effector secretion and fun-gal pathogenicity. <bold> Taken together</bold>, we discovered an unconventional protein and membrane trafficking route starting from the fungal endolysosomes to the M. oryzae-rice interaction interface and dissect the role of MoRab7/Retromer/MoSnc1 sorting machinery in effector secretion during biotro-phy and invasive growth in rice blast fungus.

引用

页码：1384 / 1403

页数：20