Polishing De Novo Nanopore Assemblies of Bacteria and Eukaryotes With FMLRC2

被引：17

作者：

Mak, Q. X. Charles ^{[1
]}

Wick, Ryan R. ^{[2
]}

Holt, James Matthew ^{[3
]}

Wang, Jeremy R. ^{[4
]}

机构：

[1] Natl Univ Singapore, Dept Biol Sci, Singapore, Singapore

[2] Univ Melbourne, Ctr Pathogen Genom, Melbourne, Australia

[3] Pacific Biosci Inc, Menlo Pk, CA USA

[4] Univ North Carolina Chapel Hill, Dept Genet, Chapel Hill, NC 27599 USA

来源：

MOLECULAR BIOLOGY AND EVOLUTION | 2023年 / 40卷 / 03期

基金：

美国国家卫生研究院;

关键词：

de novo assembly; polishing; nanopore; GENOME; TOOL;

D O I：

10.1093/molbev/msad048

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

As the accuracy and throughput of nanopore sequencing improve, it is increasingly common to perform long-read first de novo genome assemblies followed by polishing with accurate short reads. We briefly introduce FMLRC2, the successor to the original FM-index Long Read Corrector (FMLRC), and illustrate its performance as a fast and accurate de novo assembly polisher for both bacterial and eukaryotic genomes.

引用

页数：5