Effects of selenium nanoparticles produced by Lactobacillus acidophilus HN23 on lipid deposition in WRL68 cells

被引：2

作者：

Lei, Xianglan ^{[1
,2
,3
]}

Peng, Yuxuan ^{[1
,2
,3
,4
]}

Li, Yan ^{[5
]}

Chen, Qianyuan ^{[1
,2
]}

Shen, Zhenguo ^{[3
]}

Yin, Wen ^{[6
]}

Lemiasheuski, Viktar ^{[5
,7
]}

Xu, Siyang ^{[1
,2
]}

He, Jin ^{[1
,2
]}

机构：

[1] Huazhong Agr Univ, Natl Key Lab Agr Microbiol, Wuhan 430070, Peoples R China

[2] Huazhong Agr Univ, Hubei Hongshan Lab, Wuhan 430070, Peoples R China

[3] Hainan Coll Vocat & Tech, Coll Trop Agr & Technol, Haikou 570100, Hainan, Peoples R China

[4] Belarusian State Univ, Fac Biol, Minsk 220030, BELARUS

[5] Belarusian State Univ, Int Sakharov Environm Inst, Minsk 220030, BELARUS

[6] Hubei Univ, Sch Life Sci, State Key Lab Biocatalysis & Enzyme Engn, Hubei Key Lab Ind Biotechnol, Wuhan 430062, Peoples R China

[7] All Russian Res Inst Physiol Biochem & Nutr Anim, Branch Fed Res Ctr Anim Husb, Borovsk 249013, Russia

来源：

BIOORGANIC CHEMISTRY | 2024年 / 145卷

基金：

海南省自然科学基金; 中国国家自然科学基金;

关键词：

Selenium nanoparticles (SeNPs); Non-alcoholic fatty liver disease (NAFLD); Lactobacillus acidophilus; Lipid deposition; Oxidative damage; Antioxidant; LIVER; CASEI;

D O I：

10.1016/j.bioorg.2024.107165

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Selenium is an essential trace element for most organisms, protecting cells from oxidative damage caused by free radicals and serving as an adjunctive treatment for non-alcoholic fatty liver disease (NAFLD). In this study, We used the lactic acid bacterium Lactobacillus acidophilus HN23 to reduce tetra-valent sodium selenite into particulate matter, and analyzed it through inductively coupled plasma mass spectrometry (ICP-MS), scanning electron microscopy (SEM), X-ray diffraction energy dispersive spectrometry (EDS), and Fourier transform infrared spectroscopy (FTIR). We found that it consisted of selenium nanoparticles (SeNPs) with a mass composition of 65.8 % zero-valent selenium and some polysaccharide and polypeptide compounds, with particle sizes ranging from 60 to 300 nm. We also detected that SeNPs were much less toxic to cells than selenite. We further used free fatty acids (FFA)-induced WRL68 fatty liver cell model to study the therapeutic effect of SeNPs on NAFLD. The results show that SeNPs are more effective than selenite in reducing lipid deposition, increasing mitochondrial membrane potential (MMP) and antioxidant capacity of WRL68 cells, which is attributed to the chemical valence state of selenium and organic composition in SeNPs. In conclusion, SeNPs produced by probiotics L. acidophilus had the potential to alleviate NAFLD by reducing hepatocyte lipid deposition and oxidative damage. This study may open a new avenue for SeNPs drug development to treat NAFLD.

引用

页数：9