Repurposing a long-wavelength fluorescent boronate probe for the detection of reactive oxygen species (ROS) in bacteria

被引：4

作者：

Yan, Kai-Cheng ^{[1
,4
]}

Thet, Naing ^{[1
]}

Heylen, Rachel A. ^{[1
]}

Sedgwick, Adam C. ^{[2
]}

James, Tony D. ^{[1
,3
]}

Jenkins, A. Toby A. ^{[1
]}

He, Xiao-Peng ^{[4
,5
]}

机构：

[1] Univ Bath, Dept Chem, Bath BA2 7AY, Avon, England

[2] Univ Oxford, Chem Res Lab, Mansfield Rd, Oxford OX1 3TA, England

[3] Henan Normal Univ, Sch Chem & Chem Engn, Xinxiang 453007, Peoples R China

[4] East China Univ Sci & Technol, Feringa Nobel Prize Scientist Joint Res Ctr, Sch Chem & Mol Engn, Key Lab Adv Mat & Joint Int Res Lab Precis Chem, 130 Meilong Rd, Shanghai 200237, Peoples R China

[5] Eastern Hepatobiliary Surg Hosp, Natl Ctr Liver Canc, Int Cooperat Lab Signal Transduct, Shanghai 200438, Peoples R China

来源：

SENSORS & DIAGNOSTICS | 2023年 / 2卷 / 05期

基金：

英国工程与自然科学研究理事会; 美国国家科学基金会; 中国国家自然科学基金;

关键词：

DUAL-CHANNEL PROBE; OXIDATIVE STRESS; DICYANOMETHYLENE-4H-PYRAN; PEROXYNITRITE; SENSOR;

D O I：

10.1039/d3sd00049d

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Fluorescent probes are extensively used with ever-increasing functions for biological and medical applications. To further solve problems associated with tissue damage, long-wavelength fluorophores have been proposed for the construction of sensors with decreased energy requirements for activation and increased biocompatibility. As such we have repurposed an effective and biologically appliable probe TCF-Bpin combining boronate activating groups and a TCF-OH fluorophore, for monitoring the production of peroxynitrite (ONOO-) by bacteria upon treatment with antibiotics. In addition, externally added ONOO- was intracellularly visualized, which makes not only the detection of oxidative stress in bacteria possible but also increases the understanding of the mechanisms. A TCF boronate based probe developed for cellular imaging was repurposed to detect ROS in bacteria.

引用

页码：1181 / 1185

页数：5

共 19 条

[1] Oxidative stress involved in the antibacterial action of different antibiotics [J].