In vitro-in vivo wound healing efficacy of Tridax Procumbens extract loaded Carboxymethylcellulose film

In this work, Tridax Procumbens Extracts (TPE) were blended with Carboxymethylcellulose (CMC), and film was developed through the casting method. The phytochemical screening of the TPE/CMC film was carried out and found the presence of carbohydrates, tannins, saponins, and cardiac glycosides. The presence of elements such as C, O, Na, P, Cl, K, Ca, Mn, and Nb in TP/CMC film was confirmed through EDX. The melting point of the film was found around 291 +/- 0.5 degrees C which was determined through the DSC curve. The maximum tensile strength of the TPE/CMC was found as 14 +/- 0.5 MPa. The film showed antibacterial activity against Escherichia coli, Strepococcus pyogenes, Staphylococcus aureus, and Vibrio cholera compared to the control. Cell viability study exhibited 95 % and 98 % cell proliferation for the test film after interacting with the L929 cell line for 18 h and 24 h. The optical contact angle of the TPE/CMC film was also determined. The in-vivo, wound healing studies on adult mice showed healing within 10 days only and the histopathological results revealed the maximum number of fibro-blasts with a high density of collagen fibers in the test group indicating that the prepared film can be an effective wound dressing material.