Long noncoding RNA Pvt1 promotes the proliferation and migration of Schwann cells by sponging microRNA-214 and targeting c-Jun following peripheral nerve injury

被引:10
作者
Pan, Bin [1 ]
Guo, Di [1 ]
Jing, Li [2 ]
Li, Ke [3 ]
Li, Xin [1 ]
Li, Gen [1 ]
Gao, Xiao [1 ]
Li, Zhi-Wen [4 ]
Zhao, Wei [5 ]
Feng, Hu [1 ]
Cao, Meng-Han [6 ]
机构
[1] Xuzhou Med Univ, Affiliated Hosp, Dept Orthoped, Xuzhou, Jiangsu, Peoples R China
[2] Xuzhou Cent Hosp, Dept Imaging, Xuzhou, Jiangsu, Peoples R China
[3] Xuzhou Med Univ, Coll Extended Educ, Xuzhou, Jiangsu, Peoples R China
[4] Kuitun Hosp, Dept Orthoped, Yili Kazak, Xinjiang, Peoples R China
[5] Xuzhou Med Univ, Affiliated Hosp, Dept Orthoped, Xuzhou, Jiangsu, Peoples R China
[6] Xuzhou Med Univ, Affiliated Hosp, Ctr Clin Oncol, Xuzhou, Jiangsu, Peoples R China
基金
中国国家自然科学基金;
关键词
DORSAL-ROOT GANGLION; REPAIR;
D O I
10.4103/1673-5374.353497
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Research has shown that long-chain noncoding RNAs (lncRNAs) are involved in the regulation of a variety of biological processes, including peripheral nerve regeneration, in part by acting as competing endogenous RNAs. c-Jun plays a key role in the repair of peripheral nerve injury. However, the precise underlying mechanism of c-Jun remains unclear. In this study, we performed microarray and bioinformatics analysis of mouse crush-injured sciatic nerves and found that the lncRNA Pvt1 was overexpressed in Schwann cells after peripheral nerve injury. Mechanistic studies revealed that Pvt1 increased c-Jun expression through sponging miRNA-214. We overexpressed Pvt1 in Schwann cells cultured in vitro and found that the proliferation and migration of Schwann cells were enhanced, and overexpression of miRNA-214 counteracted the effects of Pvt1 overexpression on Schwann cell proliferation and migration. We conducted in vivo analyses and injected Schwann cells overexpressing Pvt1 into injured sciatic nerves of mice. Schwann cells overexpressing Pvt1 enhanced the regeneration of injured sciatic nerves following peripheral nerve injury and the locomotor function of mice was improved. Our findings reveal the role of lncRNAs in the repair of peripheral nerve injury and highlight lncRNA Pvt1 as a novel potential treatment target for peripheral nerve injury.
引用
收藏
页码:1147 / 1153
页数:7
相关论文
共 41 条
[1]   c-Jun Reprograms Schwann Cells of Injured Nerves to Generate a Repair Cell Essential for Regeneration [J].
Arthur-Farraj, Peter J. ;
Latouche, Morwena ;
Wilton, Daniel K. ;
Quintes, Susanne ;
Chabrol, Elodie ;
Banerjee, Annbily ;
Woodhoo, Ashwin ;
Jenkins, Billy ;
Rahman, Mary ;
Turmaine, Mark ;
Wicher, Grzegorz K. ;
Mitter, Richard ;
Greensmith, Linda ;
Behrens, Axel ;
Raivich, Gennadij ;
Mirsky, Rhona ;
Jessen, Kristjan R. .
NEURON, 2012, 75 (04) :633-647
[2]   Long non-coding RNA PVT1 promotes tumor progression by regulating the miR-143/HK2 axis in gallbladder cancer [J].
Chen, Jianan ;
Yu, Yan ;
Li, Hua ;
Hu, Qiuyue ;
Chen, Xiaolong ;
He, Yuting ;
Xue, Chen ;
Ren, Fang ;
Ren, Zhigang ;
Li, Juan ;
Liu, Liwen ;
Duan, Zhenfeng ;
Cui, Guangying ;
Sun, Ranran .
MOLECULAR CANCER, 2019, 18 (1)
[3]   Schwann Cell-Like Cells Derived from Human Amniotic Mesenchymal Stem Cells Promote Peripheral Nerve Regeneration through a MicroRNA-214/c-Jun Pathway [J].
Chen, Wei ;
Xiao, Shune ;
Wei, Zairong ;
Deng, Chengliang ;
Nie, Kaiyu ;
Wang, Dali .
STEM CELLS INTERNATIONAL, 2019, 2019
[4]   Silencing of Long Non-coding RNA GAS5 Suppresses Neuron Cell Apoptosis and Nerve Injury in Ischemic Stroke Through Inhibiting DNMT3B-Dependent MAP4K4 Methylation [J].
Deng, Yiming ;
Chen, Duanduan ;
Gao, Feng ;
Lv, Hong ;
Zhang, Guojun ;
Sun, Xuan ;
Liu, Lian ;
Mo, Dapeng ;
Ma, Ning ;
Song, Ligang ;
Huo, Xiaochuan ;
Yan, Tianyi ;
Zhang, Jingbo ;
Luo, Yun ;
Miao, Zhongrong .
TRANSLATIONAL STROKE RESEARCH, 2020, 11 (05) :950-966
[5]   The ARRIVE guidelines 2.0: Updated guidelines for reporting animal research [J].
du Sert, Nathalie Percie ;
Hurst, Viki ;
Ahluwalia, Amrita ;
Alam, Sabina ;
Avey, Marc T. ;
Baker, Monya ;
Browne, William J. ;
Clark, Alejandra ;
Cuthill, Innes C. ;
Dirnagl, Ulrich ;
Emerson, Michael ;
Garner, Paul ;
Holgate, Stephen T. ;
Howells, David W. ;
Karp, Natasha A. ;
Lazic, Stanley E. ;
Lidster, Katie ;
MacCallum, Catriona J. ;
Macleod, Malcolm ;
Pearl, Esther J. ;
Petersen, Ole H. ;
Rawle, Frances ;
Reynolds, Penny ;
Rooney, Kieron ;
Sena, Emily S. ;
Silberberg, Shai D. ;
Steckler, Thomas ;
Wuerbel, Hanno .
PLOS BIOLOGY, 2020, 18 (07)
[6]   LncRNA PVT1 Facilitates Tumorigenesis and Progression of Glioma via Regulation of MiR-128-3p/GREM1 Axis and BMP Signaling Pathway [J].
Fu, Chao ;
Li, Dongyuan ;
Zhang, Xiaonan ;
Liu, Naijie ;
Chi, Guonan ;
Jin, Xingyi .
NEUROTHERAPEUTICS, 2018, 15 (04) :1139-1157
[7]   LncRNA BC083743 Promotes the Proliferation of Schwann Cells and Axon Regeneration Through miR-103-3p/BDNF After Sciatic Nerve Crush [J].
Gao, Lin ;
Feng, Aiqin ;
Yue, Peijian ;
Liu, Yue ;
Zhou, Qiaoyu ;
Zang, Qiuling ;
Teng, Junfang .
JOURNAL OF NEUROPATHOLOGY AND EXPERIMENTAL NEUROLOGY, 2020, 79 (10) :1100-1114
[8]  
He XZ, 2016, ZHONGGUO ZUZHI GONGC, V20, P1044
[9]   The repair Schwann cell and its function in regenerating nerves [J].
Jessen, K. R. ;
Mirsky, R. .
JOURNAL OF PHYSIOLOGY-LONDON, 2016, 594 (13) :3521-3531
[10]   Schwann Cells: Development and Role in Nerve Repair [J].
Jessen, Kristjan R. ;
Mirsky, Rhona ;
Lloyd, Alison C. .
COLD SPRING HARBOR PERSPECTIVES IN BIOLOGY, 2015, 7 (07) :1-15