Effect of the micro/nanostructured topography of polyetheretherketone on the behavior of MC3T3-E1 preosteoblasts

被引：0

作者：

Zhao, Yuqing ^{[1
,2
]}

Wang, Jing ^{[3
]}

Wu, Fan ^{[4
]}

Liu, Lipeng ^{[5
]}

Wu, Gaoyi ^{[1
,6
,7
,8
]}

Zhou, Libo ^{[1
,2
,7
,8
]}

机构：

[1] Jiamusi Univ, Heilongjiang Key Lab Oral Biomed Mat & Clin Applic, Jiamusi, Peoples R China

[2] Jiamusi Univ, Affiliated Stomatol Hosp, Expt Ctr Stomatol Engn, Dept Stomatol, Jiamusi, Peoples R China

[3] Fourth Mil Med Univ, Shaanxi Engn Res Ctr Dent Mat & Adv Manufacture, State Key Lab Oral & Maxillofacial Reconstruct & R, Natl Clin Res Ctr Oral Dis,Dept Oral Implants, Xian, Peoples R China

[4] Jiamusi Univ, Key Lab Microecol Immune Regulatory Network & Rela, Sch Basic Med, Jiamusi, Peoples R China

[5] Fourth Mil Med Univ, State Key Lab Oral & Maxillofacial Reconstruct & R, Natl Clin Res Ctr Oral Dis, Shaanxi Clin Res Ctr Oral Dis,Dept Pediat Dent,Sch, Xian, Peoples R China

[6] Jiamusi Univ, Affiliated Stomatol Hosp, Expt Ctr Stomatol Engn, Jiamusi, Peoples R China

[7] Jiamusi Univ, Heilongjiang Key Lab Oral Biomed Mat & Clin Applic, Jiamusi 154000, Peoples R China

[8] Jiamusi Univ, Affiliated Stomatol Hosp, Expt Ctr Stomatol Engn, Jiamusi 154000, Peoples R China

来源：

JOURNAL OF APPLIED POLYMER SCIENCE | 2024年 / 141卷 / 15期

基金：

中国国家自然科学基金;

关键词：

micro/nanostructured topography; polyetheretherketone; preosteoblasts; sulfonation; SURFACE-TOPOGRAPHY; BONE-FORMATION; STEM-CELLS; OSTEOBLAST; TITANIUM; PEEK; OSSEOINTEGRATION; DIFFERENTIATION; PHOSPHORYLATION; PROLIFERATION;

D O I：

10.1002/app.55214

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Polyetheretherketone (PEEK) is of interest because of its excellent mechanical properties. However, the bioinert nature of PEEK limits its use in clinical applications. In this study, a series of micro/nanostructures combining nano-, submicron-, and microscale on PEEK were fabricated with 0.5, 1, 4, and 6 min of sulfonation time (S1, S2, S3, and S4). Compared to the flat surface on PEEK, the micro/nanostructure of different sizes all significantly promote cell adhesion, proliferation, and osteogenic differentiation of MC3T3-E1 cells. It is shown that micro/nano-porous structures with smaller size and lower roughness of S1 enabled faster cell propagation. The results of alkaline phosphatase staining, alizarin red staining, and quantitative real-time PCR reveal that the osteogenic activity of MC3T3-E1 cells gradually decreases with the increasement of pore size, indicating that the micro/nanostructured topography of S1 generated substantially increased matrix mineralization and bone-like nodule formation, compared to the 3D network surface. This work provides an effective strategy for designing biomaterials with potential clinical applications.

引用

页数：11