Hand sanitiser activity test of eucalyptus (Eucalyptus globulus) oil extract against Bacillus subtilis and Enterococcus faecalis bacteria

Background: Bacillus subtillis bacteria can cause meningitis, endocarditis, and eye infections, while Enterococcus can cause meningitis in neonates and endocarditis in adults. Therefore, it is important to use hand sanitiser to avoid bacterial infections. Eucalyptus is an essential oil-producing plant that can inhibit growth and kill bacteria with its compounds, namely linalool and pinocarveol. Purpose: To make a good eucalyptus oil hand sanitiser and test its effectiveness against Bacillus subtilis and Enterococcus faecalis bacteria. Method: Making transparent hand sanitiser with a mixture of eucalyptus oil extract. Physical, chemical and antibacterial tests on Bacillus subtilis and Enterococcus faecalis. Results: The hand sanitiser preparation obtained an acidity of 7, with a specific gravity of 0.8512 g/ml, and a viscosity of 4.14 cP and the cleaning power of Bacillus subtilis and Enterococcus faecalis was 99.9%. Conclusion: Eucalyptus oil extract hand sanitiser is of good quality and effectively kills Bacillus subtilis and Enterococcus faecalis bacteria.