Anti-microbial, anti-oxidant and wound healing capabilities of Aloe vera-incorporated hybrid nanoflowers

被引:24
作者
Altinkaynak, Cevahir [1 ]
Haciosmanoglu, Ebru [2 ]
Ekremoglu, Murat [3 ]
Hacioglu, Mayram [4 ]
Ozdemir, Nalan [5 ]
机构
[1] Nevsehir Haci Bektas Veli Univ, Avanos Vocat Sch, Dept Plant & Anim Prod, TR-50500 Nevsehir, Turkiye
[2] Bezmialem Vakif Univ, Fac Med, Dept Biophys, TR-34093 Istanbul, Turkiye
[3] Istinye Univ, Fac Med, Dept Med Biochem, TR-34010 Istanbul, Turkiye
[4] Istanbul Univ, Fac Pharm, Dept Pharmaceut Microbiol, TR-34116 Istanbul, Turkiye
[5] Erciyes Univ, Fac Sci, Dept Chem, TR-38039 Kayseri, Turkiye
关键词
Hybrid nanoflower; Aloe vera; Wound healing; Anti-microbial; Anti-oxidant; SILVER NANOPARTICLES; GREEN SYNTHESIS; METAL-IONS; BARBADENSIS; EXTRACT; L; BIOSYNTHESIS; PLANTS; GEL;
D O I
10.1016/j.jbiosc.2023.01.004
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
The active ingredients of Aloe vera have attracted attention for their potential use in nanotechnology-based medical applications and biomaterial production. It has many therapeutic applications in modern world. This study used Aloe vera extract in different concentrations to synthesize Aloe vera-incorporated hybrid nanoflowers (AV-Nfs). The most uniform morphology in the nanoflowers obtained was at a concentration of 2 mL. The AV-Nfs were well characterized by scanning electron microscopy, X-ray spectroscopy, Fourier transform infrared spectroscopy, and X-ray diffraction (XRD). The highest peroxidase-mimicking activity of the components was 1.488 EU/mg at 60 degrees C and pH 6. The DPPH assay determined the antioxidant activity of the components and the MTT assay tested on CCD-1072Sk fibroblast cell line determined the effect of AV-Nfs on cell proliferation. Separate treatment of AV-Nfs with Cu3(PO4)2 center dot 3H2O significantly increased cell proliferation according to free Aloe vera and CuSO4. In vitro wound healing results showed that AV-Nfs could significantly close wounds compared to free Aloe vera. In this study, AV-Nfs showed antimicrobial activity against Staphylococcus epidermidis, Enterococcus faecalis, Escherichia coli and Klebsiella pneumoniae at minimum inhibitory concentration of 625 mg/mL, suggesting that AV-Nfs may be used in wound healing applications with enhanced bio-logical properties. AV-Nfs showed no activity against the yeast Candida albicans.(c) 2023, The Society for Biotechnology, Japan. All rights reserved.
引用
收藏
页码:321 / 330
页数:10
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