Mechanism of HCG regulating epithelial-mesenchymal transition progression in endometrial cells through the FoxO1/miR223-5p/Wnt5a pathway

被引:1
作者
Wu, Shuxie [1 ]
Wu, Gao [2 ]
Li, Yanping [3 ]
Wu, Hanbin [4 ]
机构
[1] Fudan Univ, Obstet & Gynaecol Hosp, Shanghai, Peoples R China
[2] Naval Mil Med Univ, Dept Pharm, Affiliated Hosp 1, Shanghai, Peoples R China
[3] Cent South Univ, Xiangya Hosp, Reprod Med Ctr, Changsha, Hunan, Peoples R China
[4] Tongji Univ, Sch Med, Shanghai East Hosp, Clin Pharm, Shanghai, Peoples R China
基金
中国国家自然科学基金;
关键词
Human chorionic gonadotrophin; EMT; Wnt5a; FoxO1; FoxO1 Endometrial receptivity; miR223-5p;
D O I
10.1016/j.rbmo.2023.05.016
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Research question: Does human chorionic gonadotrophin (HCG) influence endometrial receptivity and epithelial-mesenchymal transition (EMT) via the FoxO1/miR223-5p/Wnt5a pathway? Design: This study aimed to establish the co -culture system of human embryonic trophoblast cell line (HTR-8-Svneo) cells and human endometrial epithelial cell line (HEEC) cells. The expression of Wnt5a protein and EMT -related proteins in HTR-8-Svneo and HEEC cells treated in a gradient -dependent manner using HCG and exosome inhibitor GW4869 were detected in the coculture system. Results: In the HTR-8-Svneo/HEEC co -culture system, miR223-5p in HEEC cells increased significantly with induction of HTR-8Svneo cells by 100 IU/ml HCG for 48 h (P = 0.046), and Wnt5a protein decreased significantly in HEEC cells (P = 0.021). Pretreatment of HTR-8-Svneo cells with GW4869, and knockdown of FoxO1 in HTR-8-Svneo cells, significantly inhibited the above effects of HCG on miR223-5p and Wnt5a expression in HEEC cells in the HTR-8-Svneo/HEEC co -culture system. HTR-8Svneo cells induced with 100 IU/ml HCG for 48 h significantly enhanced the logarithmic phase proliferation activity of HEEC cells in the co -culture system (P < 0.001), while knockdown of FoxO1 in HTR-8-Svneo cells and inhibition of miR223-5p in HEEC cells suppressed proliferation of HEEC cells in the HTR-8-Svneo/HEEC co -culture system (P < 0.001). Conclusions: HCG exposure induces HTR-8-Svneo cells to up -regulate miR223-5p expression, which enters HEEC cells in the co -culture system through the exosomal pathway, and inhibits Wnt5a expression and the progress of EMT.
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页数:12
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