High-risk pathological subtype associated FAM83A-AS1 promotes malignancy and glycolysis of lung adenocarcinoma via miR-202-3p/HK2 axis

被引:3
作者
Xiong, Xinkui [1 ]
Zhang, Lei [2 ]
Zang, Bao [1 ]
Xu, Dafu [1 ]
Chen, Chen [1 ]
Dong, Gaochao [3 ,4 ,5 ]
Xia, Wenjie [3 ,4 ,5 ]
Wu, Yanhu [6 ,7 ]
机构
[1] Nanjing Med Univ, Dept Thorac Surg, Affiliated Huaian Peoples Hosp 1, Huaian 223001, Jiangsu, Peoples R China
[2] Xuzhou Cent Hosp, Dept Med Oncol, Xuzhou 221000, Peoples R China
[3] Nanjing Med Univ, Dept Thorac Surg, Affiliated Canc Hosp, Nanjing, Peoples R China
[4] Jiangsu Canc Hosp, Nanjing, Peoples R China
[5] Jiangsu Inst Canc Res, Nanjing, Peoples R China
[6] Nanjing Med Univ, Dept Cardiovasc Surg, Affiliated Hosp 1, Nanjing 210000, Jiangsu, Peoples R China
[7] Nanjing Med Univ, Dept Cardiovasc Surg, Affiliated Hosp 1, 300 Guangzhou Rd, Nanjing 210000, Jiangsu, Peoples R China
关键词
lung adenocarcinoma; FAM83A-AS1; histological pathology subtype; hexokinase II; glycolysis; LONG NONCODING RNA; AEROBIC GLYCOLYSIS; CANCER; MIGRATION; CHEMORESISTANCE; PROLIFERATION; PROGRESSION; SIGNATURE; FEATURES; TARGETS;
D O I
10.3892/or.2023.8532
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
According to the diverse cellular morphology, lung adenocarcinoma (LUAD) was classified into five pathological subtypes, referred to as follows: High-risk group (micropapillary and solid), intermediate-risk group (acinar and papillary) and low-risk group (epidic). Nevertheless, little is known about the biological function of long non-coding RNA (lncRNA) in the molecular determination of LUAD histologic patterns. Screening the transcriptional expression data from TCGA-LUAD, the differentially expressed lncRNA across the divergent pathological subtypes were explored. Pan-cancer analysis revealed the characteristic of FAM83A-AS1, which was also confirmed in the LUAD tissues. The function of FAM83A-AS1 was uncovered through the in vitro assays. RNA immunoprecipitation and dual-luciferase reporter assays were performed to explore the molecular mechanisms of FAM83A-AS1. In the present study, it was identified that the expression of FAM83A-AS1 was increased from the low-risk group to the high, which was associated with a poorer prognosis and higher risk of recurrence. Pan-cancer analysis revealed that FAM83A-AS1 was positively correlated with high tumor mutational burden. Additionally, FAM83A-AS1 promoted cell migration, invasion and growth of LUAD cancer cells. Mechanistically, FAM83A-AS1 sponged miR-202-3p to regulate the expression of hexokinase II (HK2) in post-transcription, which facilitated the malignancy and glycolysis. The present study uncovered the biological roles of FAM83A-AS1/miR-202-3p/HK2 axis in regulating malignancy and glycolysis of LUAD, which provided novel avenues to addressing the determination of histologic patterns.
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页数:14
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