Establishment of Dendrobium wilsonii Rolfe in vitro regeneration system

Tissue culture processes have been established for Dendrobium wilsonii Rolfe, a valued ornamental and medicinal orchid. Pods aged 180 days served as seed sources. These seeds underwent surface sterilization and were then aseptically germinated on Murashige and Skoog (MS) medium supplemented with 0.5 mg L-1 Benzyl adenine (BA), 0.5 mg L-1 Naphthalene acetic acid (NAA), 20 g L-1 Sucrose (Su), 7 g L-1 Agar, 1 g L-1 Activated charcoal (AC), 1 g L-1 Peptone and 1 g L-1 Hyponex No.1 incubated in a growth chamber at 25.2 degrees C with a 12-hour photoperiod and a light intensity of 2200 lx. Multiple shoot induction and proliferation was on MS medium in vitro produced shoots were rooted on MS medium with Hyponex No.1, 25 g L-1 Su, 1 g L-1 AC and 1.5 g L-1 Peptone. Peptone enhanced seedling growth and promoted clumped shoot development. The establishment of Dendrobium wilsonii Rolfe regeneration system provides a methodology for conserving germplasm resources and for commercial propagules production.