In Planta Arsenic Thiolation in Rice and Arabidopsis thaliana

被引:2
作者
Blanco, Andrea E. Colina [1 ]
Pischke, Erik [2 ]
Mori, Alejandra Higa [1 ]
Kerl, Carolin F. [1 ]
Clemens, Stephan [2 ]
Planer-Friedrich, Britta [1 ]
机构
[1] Univ Bayreuth, Bayreuth Ctr Ecol & Environm Res BAYCEER, Environm Geochem Grp, D-95440 Bayreuth, Germany
[2] Univ Bayreuth, Bayreuth Ctr Ecol & Environm Res BayCEER, Plant Physiol, D-95440 Bayreuth, Germany
关键词
Oryza sativa; arsenic speciation; DMMTA; DMDTA; glutathione; food safety; DIMETHYLMONOTHIOARSINIC ACID; ACCUMULATION; GRAIN; TRANSLOCATION; LOCALIZATION; GLUTATHIONE; METABOLISM; SPECIATION; TRANSPORT; TOXICITY;
D O I
10.1021/acs.est.3c06603
中图分类号
X [环境科学、安全科学];
学科分类号
08 ; 0830 ;
摘要
Inorganic and methylated thioarsenates have recently been reported to form in paddy soil pore waters and accumulate in rice grains. Among them, dimethylmonothioarsenate (DMMTA) is particularly relevant because of its high cytotoxicity and potential misidentification as nonregulated dimethylarsenate (DMA). Studying DMMTA uptake and flag leaf, grain, and husk accumulation in rice plants during grain filling, substantial dethiolation to DMA was observed with only 8.0 +/- 0.1, 9.1 +/- 0.6, and 1.4 +/- 0.2% DMMTA remaining, respectively. More surprisingly, similar shares of DMMTA were observed in control experiments with DMA, indicating in planta DMA thiolation. Exposure of different rice seedling varieties to not only DMA but also to arsenite and monomethylarsenate (MMA) revealed in planta thiolation as a common process in rice. Up to 35 +/- 7% DMA thiolation was further observed in the shoots and roots of the model plant Arabidopsis thaliana. Parameters determining the ratio and kinetics of thiolation versus dethiolation are unknown, yet, but less DMA thiolation in glutathione-deficient mutants compared to wild-type plants suggested glutathione concentration as one potential parameter. Our results demonstrate that pore water is not the only source for thioarsenates in rice grains and that especially the currently nonregulated DMA needs to be monitored as a potential precursor of DMMTA formation inside rice plants.
引用
收藏
页码:21846 / 21854
页数:9
相关论文
共 51 条
  • [1] Comparison of a chemical and enzymatic extraction of arsenic from rice and an assessment of the arsenic absorption from contaminated water by cooked rice
    Ackerman, AH
    Creed, PA
    Parks, AN
    Fricke, MW
    Schwegel, CA
    Creed, JT
    Heitkemper, DT
    Vela, NP
    [J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2005, 39 (14) : 5241 - 5246
  • [2] Widespread occurrence of dimethylmonothioarsenate (DMMTA) in rice cakes: Effects of puffing and storage
    Blanco, Andrea E. Colina
    Mori, Alejandra Higa
    Planer-Friedrich, Britta
    [J]. FOOD CHEMISTRY, 2024, 436
  • [3] Detection of Thioarsenates in Rice Grains and Rice Products
    Blanco, Andrea E. Colina
    Kerl, Carolin F.
    Planer-Friedrich, Britta
    [J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2021, 69 (07) : 2287 - 2294
  • [4] Knocking Out OsPT4 Gene Decreases Arsenate Uptake by Rice Plants and Inorganic Arsenic Accumulation in Rice Grains
    Cao, Yue
    Sun, Dan
    Ai, Hao
    Mei, Hanyi
    Liu, Xue
    Sun, Shubin
    Xu, Guohua
    Liu, Yungen
    Chen, Yanshan
    Ma, Lena Q.
    [J]. ENVIRONMENTAL SCIENCE & TECHNOLOGY, 2017, 51 (21) : 12131 - 12138
  • [5] Phloem transport of arsenic species from flag leaf to grain during grain filling
    Carey, Anne-Marie
    Norton, Gareth J.
    Deacon, Claire
    Scheckel, Kirk G.
    Lombi, Enzo
    Punshon, Tracy
    Guerinot, Mary Lou
    Lanzirotti, Antonio
    Newville, Matt
    Choi, Yongseong
    Price, Adam H.
    Meharg, Andrew A.
    [J]. NEW PHYTOLOGIST, 2011, 192 (01) : 87 - 98
  • [6] Grain Unloading of Arsenic Species in Rice
    Carey, Anne-Marie
    Scheckel, Kirk G.
    Lombi, Enzo
    Newville, Matt
    Choi, Yongseong
    Norton, Gareth J.
    Charnock, John M.
    Feldmann, Joerg
    Price, Adam H.
    Meharg, Andrew A.
    [J]. PLANT PHYSIOLOGY, 2010, 152 (01) : 309 - 319
  • [7] Sulfate-reducing bacteria and methanogens are involved in arsenic methylation and demethylation in paddy soils
    Chen, Chuan
    Li, Lingyan
    Huang, Ke
    Zhang, Jun
    Xie, Wan-Ying
    Lu, Yahai
    Dong, Xiuzhu
    Zhao, Fang-Jie
    [J]. ISME JOURNAL, 2019, 13 (10) : 2523 - 2535
  • [8] Toxic Heavy Metal and Metalloid Accumulation in Crop Plants and Foods
    Clemens, Stephan
    Ma, Jian Feng
    [J]. ANNUAL REVIEW OF PLANT BIOLOGY, VOL 67, 2016, 67 : 489 - 512
  • [9] The glutathione-deficient, cadmium-sensitive mutant, cad2-1, of Arabidopsis thaliana is deficient in γ-glutamylcysteine synthetase.
    Cobbett, CS
    May, MJ
    Howden, R
    Rolls, B
    [J]. PLANT JOURNAL, 1998, 16 (01) : 73 - 78
  • [10] Methylated and thiolated arsenic species for environmental and health research - A review on synthesis and characterization
    Cullen, William R.
    Liu, Qingqing
    Lu, Xiufen
    McKnight-Whitford, Anthony
    Peng, Hanyong
    Popowich, Aleksandra
    Yan, Xiaowen
    Zhang, Qi
    Fricke, Michael
    Sun, Hongsui
    Le, X. Chris
    [J]. JOURNAL OF ENVIRONMENTAL SCIENCES, 2016, 49 : 7 - 27