The Separation, Purification, Structure Identification, and Antioxidant Activity of Elaeagnus umbellata Polysaccharides

被引：12
作者：
Zhang, Jinhua [1 ,2 ]
Xu, Xin [1 ,2 ]
Liu, Xinyi [1 ,2 ]
Chen, Min [3 ]
Bai, Baoqing [1 ,2 ]
Yang, Yukun [1 ,2 ]
Bo, Tao [4 ]
Fan, Sanhong [1 ,2 ]
机构：
[1] Shanxi Univ, Sch Life Sci, Taiyuan 030006, Peoples R China
[2] Shanxi Univ, Shanxi Key Lab Res & Dev Reg Plants, Taiyuan 030006, Peoples R China
[3] Shanxi Food Res Inst Co Ltd, Taiyuan 030024, Peoples R China
[4] Shanxi Univ, Inst Biotechnol, Taiyuan 030006, Peoples R China
来源：
MOLECULES | 2023年 / 28卷 / 18期
关键词：
Elaeagnus umbellata; polysaccharides; antioxidant activity; functional food; PHYSICOCHEMICAL PROPERTIES; SULFATED POLYSACCHARIDES; SOPHORAE-TONKINENSIS; EXTRACTION; OPTIMIZATION; SPECTROSCOPY; PLANT;
D O I：
10.3390/molecules28186468
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
In order to investigate the antioxidant activity of Elaeagnus umbellata polysaccharides, the physicochemical characteristics of purified Elaeagnus umbellata polysaccharides (EUP, consisting of two fractions, EUP1 and EUP2) were investigated using UV spectrophotometry, high-performance liquid chromatography (HPLC), high-performance gel permeation chromatography (HPGPC), and Fourier transform infrared spectroscopy (FT-IR). This revealed that EUP1 and EUP2 were acidic polysaccharides with an average molecular weight (MW) of 63 and 38 kDa, respectively. EUP1 mainly consisted of L-rhamnose and D-galactose in a molar ratio of 2.05:1, and EUP2 consisted of D-mannose, L-rhamnose, D-galactose, and D-arabinose in a molar ratio of 2.06:1:2.78:1. Furthermore, EUP exhibited considerable antioxidant potential for scavenging hydroxyl, superoxide anion, DPPH, and ABTS radicals. Therefore, EUP can be developed as a potential antioxidant for the functional food or pharmaceutical field.
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页数：14
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