A Two-color Single-molecule Sequencing Platform and Its Clinical Applications

被引：0

作者：

Chen, Fang ^{[1
]}

Liu, Bin ^{[1
]}

Chen, Meirong ^{[1
]}

Jiang, Zefei ^{[1
]}

Zhou, Zhiliang ^{[1
]}

Wu, Ping ^{[1
]}

Zhang, Meng ^{[1
]}

Jin, Huan ^{[1
]}

Li, Linsen ^{[1
]}

Lu, Liuyan ^{[1
]}

Shang, Huan ^{[1
]}

Liu, Lei ^{[1
]}

Chen, Weiyue ^{[1
]}

Xu, Jianfeng ^{[1
]}

Sun, Ruitao ^{[1
]}

Wang, Guangming ^{[1
]}

Zheng, Jiao ^{[1
]}

Qi, Jifang ^{[1
]}

Yang, Bo ^{[1
]}

Zeng, Lidong ^{[1
]}

Li, Yan ^{[1
]}

Lv, Hui ^{[1
]}

Zhao, Nannan ^{[1
]}

Wang, Wen ^{[1
]}

Cai, Jinsen ^{[1
]}

Liu, Yongfeng ^{[1
]}

Luo, Weiwei ^{[1
]}

Zhang, Juan ^{[1
]}

Zhang, Yanhua ^{[1
]}

Fan, Jicai ^{[1
]}

Dan, Haitao ^{[1
]}

He, Xuesen ^{[1
]}

Huang, Wei ^{[1
]}

Sun, Lei ^{[1
]}

Yan, Qin ^{[1
]}

机构：

[1] GeneMind Biosci Co Ltd, Shenzhen 518000, Peoples R China

来源：

GENOMICS PROTEOMICS & BIOINFORMATICS | 2024年 / 22卷 / 01期

关键词：

GenoCare; Single-molecule sequencing; SARS-CoV-2; Microbial quantitation; Variant detection;

D O I：

10.1093/gpbjnl/qzae006

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

DNA sequencers have become increasingly important research and diagnostic tools over the past 20 years. We have developed a single-molecule desktop sequencer, GenoCare 1600 (GenoCare), which utilizes amplification-free library preparation and two-color sequencing-by-synthesis chemistry, making it more user-friendly compared to previous single-molecule sequencing platforms for clinical use. Here, we report sequencing data of an Escherichia coli standard sample by GenoCare, with a consensus accuracy reaching 99.99%. We also evaluated the sequencing performance of this platform in microbial mixtures and coronavirus disease 2019 (COVID-19) samples from throat swabs. Our findings indicate that the GenoCare platform allows for microbial quantitation, sensitive identification of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, and accurate detection of virus mutations, as confirmed by Sanger sequencing, demonstrating remarkable potential in clinical application.

引用

页数：7

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