Aedes aegypti Totivirus identified in mosquitoes in the Brazilian Amazon region

被引：1

作者：

Leal, Elcio ^{[1
]}

D'Athaide Ribeiro, Edcelha Soares ^{[2
]}

Costa Monteiro, Fred Julio ^{[2
]}

Marques, Julia Pantoja ^{[2
]}

Mendes, Diuliana dos Santos ^{[2
]}

Morais, Vanessa S. ^{[3
]}

Lima Araujo, Emerson Luiz ^{[4
]}

Pandey, Ramendra Pati ^{[5
]}

Chang, Chung-Ming ^{[6
]}

Deng, Xutao ^{[7
,8
]}

Delwart, Eric ^{[7
,8
]}

da Costa, Antonio Charlys ^{[3
]}

Lima, Kledoaldo ^{[9
,10
,11
]}

机构：

[1] Univ Fed Para, Inst Ciencias Biol, Lab Diversidade Viral, BR-66075000 Belem, Para, Brazil

[2] Publ Hlth Lab Amapa LACEN AP, Hlth Surveillance Superintendence Amapa, BR-68905230 Macapa, Brazil

[3] Univ Sao Paulo, Fac Med, Inst Med Trop, BR-05403000 Sao Paulo, Brazil

[4] Secretariat Minist Hlth CGLAB DAEVS SVS MS, Dept Hlth Surveillance, Gen Coordinat Publ Hlth Labs Strateg Articulat, BR-70719040 Brasilia, DF, Brazil

[5] SRM Univ, Ctr Drug Design Discovery & Dev C4D, Delhi NCR, Sonepat 131029, Haryana, India

[6] Chang Gung Univ, Master & PhD Program Biotechnol Ind, 259,Wenhua 1st Rd, Taoyuan 33302, Taiwan

[7] Vitalant Res Inst, 270 Masonic Ave, San Francisco, CA 94118 USA

[8] Univ Calif San Francisco, Dept Lab Med, San Francisco, CA 94143 USA

[9] Univ Fed Pernambuco, Hosp Clin, BR-50670901 Recife, PE, Brazil

[10] European Virus Bioinformat Ctr, Leutragraben 1, D-07743 Jena, Germany

[11] Fac Pernambucana Saude, BR-51150000 Recife, PE, Brazil

来源：

VIRUS GENES | 2023年 / 59卷 / 01期

关键词：

Totivirus; Totiviridae; Aedes; Mosquitoes; Metagenomics; Virome; INSECT-SPECIFIC FLAVIVIRUSES; STRANDED-RNA VIRUS; CULEX-TRITAENIORHYNCHUS; TRANSMISSION; REPLICATION; DISCOVERY; GENUS;

D O I：

10.1007/s11262-022-01955-z

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The totiviridae family contains viruses with double-stranded RNA genomes of 4.6-7.0 kpb, which encode a capsid protein (CP) and RNA-dependent RNA polymerase (RdRp), and they are approximately 40 nm in diameter with icosahedral symmetry. Totiviruses were first isolated from mosquitoes collected in Shaanxi Province (China). Here, we report a new Aedes aegypti Totivirus (AaTV) identified in mosquitoes from the Amazon rainforest. Mosquitoes (Diptera: Culicidae) were collected from a forest reserve belonging to the Amazon forest in the city of Macapa, Amapa state, Northern Brazil. A viral sequence with a 5748 nucleotide length that was nearly identical to Aedes aegypti Totivirus (AaTV), here named Aedes aegypti Totivirus BR59AP, was detected. A detailed molecular analysis was performed and shows that AaTV-BR59AP is highly related to the AaTV strain from the Caribbean region. We emphasize the importance of the characterization of new viruses in mosquitoes to deepen our understanding of viral diversity in insects and their potential role in disease.

引用

页码：167 / 172

页数：6

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