Mesoscale convective systems (MCSs) are crucial in modifying the water cycle and frequently induce high-impact weather events over eastern China. Radar and Climate Prediction Center (CPC)-4 km satellite-derived infrared cloud top temperature (Tb) data were used to thoroughly analyze the long-term climatology of MCSs over eastern China, particularly in the Yangtze-Huaihe River Basin (YHR) in the warm season from 2013 to 2018. For the first time, we contrasted the effects of data set selection and threshold setting on research outcomes. The large-scale environments of MCSs initiation were also investigated using the latest global reanalysis data ERA5. It is found that striction of thresholds, including duration, reflectivity/Tb, area, and linearity, would lead to a greater proportion of early-morning MCSs. Satellite-identified MCSs differed from radar-derived ones, exhibiting afternoon diurnal peaks, faster movement speeds, longer travel distances, and expansive impact areas. The center of MCS and related precipitation shifted northward from Pre-Meiyu to Post-Meiyu seasons, contributing to up to 20% of total rainfall, with most MCSs moving along eastward trajectories. MCSs typically had the most substantial impact in the Meiyu season because of the most prolonged duration, largest convective core area, and strongest precipitation intensity. Warm-season MCSs initiated ahead of midlevel troughs and were related to strong anomalous low-level convergence and midlevel upward. The circulation anomalies were the strongest in the Pre-Meiyu season among the three subseasons, with most moisture sourced from the southwest. This work used radar and satellite data to analyze the climate characteristics of a kind of large thunderstorms known as mesoscale convective systems (MCSs) in the Yangtze-Huaihe River Basin (YHR) in eastern China. These systems cover large areas, lasting from a few hours to a few days. The analysis revealed that stricter tracking thresholds would lead to more early-morning MCSs. Satellite-identified MCSs differed from those identified by radar. They tended to have more thunderstorms in the afternoon, move faster, travel longer distances, and cover larger areas. MCSs also contributed to up to 20% of the total warm-season rainfall in the YHR. MCSs typically had the most substantial impact in the rainy season of eastern China from mid-June to mid-July. Warm-season MCSs initiated ahead of low-pressure systems and were related to strong anomalous low-level convergence and midlevel upward motion. During the time before the rainy season, the large-scale forcing needed to be anomaly enough to trigger MCS formation. Most of the moisture that caused MCSs came from southwest direction instead of stemming locally during this period. Six-year climatology of warm-season mesoscale convective systems (MCSs) in Yangtze-Huaihe River Basin is investigated using satellite, radar data, and ERA5Criteria setting for tracking MCSs noticeably influences the derived featuresMeiyu season MCSs featured longest duration, largest areas, and heaviest precipitation, while the background circulation anomalies are the weakest