A comparison between optimized PLGA and CS-Alg-PLGA microspheres for long-lasting release of glatiramer acetate

被引：10

作者：

Hajian, Maedeh ^{[1
,2
]}

Erfani-Moghadam, Vahid ^{[1
,3
]}

Arabi, Mehdi Sheikh ^{[1
,3
]}

Soltani, Alireza ^{[4
]}

Shahbazi, Majid ^{[1
,5
]}

机构：

[1] Golestan Univ Med Sci, Med Cellular & Mol Res Ctr, Gorgan, Iran

[2] Golestan Univ Med Sci, Sch Adv Technol Med, Dept Med Biotechnol, Gorgan, Iran

[3] Golestan Univ Med Sci, Sch Adv Technol Med, Dept Med Nanotechnol, Gorgan, Iran

[4] Golestan Univ Med Sci, Golestan Rheumatol Res Ctr, Gorgan, Iran

[5] Biopharmaceut Co, AryaTinaGene ATG, Gorgan, Iran

来源：

JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY | 2023年 / 82卷

基金：

芬兰科学院;

关键词：

Multiple sclerosis; Glatiramer acetate; PLGA; Chitosan; Alginate; Microparticles; MULTIPLE-SCLEROSIS; NANOPARTICLES; MECHANISMS; DELIVERY; CHITOSAN; DIFFERENTIATION; MICROPARTICLES; CYTOTOXICITY; SYSTEM; REDUCE;

D O I：

10.1016/j.jddst.2023.104355

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Glatiramer acetate (GA, Copaxone, COP, Copolymer-1) is one of the most prescribed drugs to treat relapsingremitting multiple sclerosis (RRMS). Herein, the formula of GA entrapped chitosan-alginate-poly (D, L-lacticco-glycolic acid) (CS-Alg-PLGA) microsphere has been studied as a novel carrier compared with conventional PLGA microsphere, pursuing long-lasting release to reduce the number of injections and minimize side effects. The preparation methods, including double-emulsion, homogenization, and solvent evaporation, were evaluated in preparing the GA/PLGA and GA/CS-Alg-PLGA microspheres. Dynamic light scattering (DLS), Field Emission Scanning Electron Microscopes (FE-SEM), Fourier transform infrared spectroscopy (FTIR), 1H nuclear magnetic resonance (1H NMR), and Thermogravimetric analysis (TGA) were performed for microspheres characterization. GA was successfully loaded within the PLGA and CS-Alg-PLGA microspheres with 96.93 +/- 0.9% and 97 +/- 0.86% encapsulation efficiency, with 96.2 +/- 1.24% and 86.91 +/- 1.18% in vitro release in 21 days, respectively. Moreover, the cell toxicity assay showed that the PLGA and CS-Alg-PLGA microspheres with and without the GA drug were nontoxic (at the range of this experiment) for Human Dermal Fibroblasts (HDF) cell line. In vitro release experiments exhibited long-lasting release of GA from both microspheres. Using the GA/PLGA and GA/ CS-Alg-PLGA microspheres in treating RRMS may improve the therapeutic efficiency and bioavailability of the drug.

引用

页数：10

共 56 条

[1] Alginate modified-PLGA nanoparticles entrapping amikacin and moxifloxacin as a novel host-directed therapy for multidrug-resistant tuberculosis [J].