Protective effects of Bifidobacterium bifidum FL-228.1 on dextran sulfate sodium-induced intestinal damage in mice

被引：8

作者：

Wang, Rui ^{[1
]}

Kuerman, Malina ^{[1
]}

Cui, Qingyu ^{[1
]}

Tian, Xiaoying ^{[1
]}

Zhou, Yu ^{[1
]}

Yi, Huaxi ^{[1
]}

Gong, Pimin ^{[1
]}

Lin, Kai ^{[1
]}

Zhang, Zhe ^{[1
]}

Liu, Tongjie ^{[1
]}

Zhang, Lanwei ^{[1
]}

机构：

[1] Ocean Univ China, Coll Food Sci & Engn, 5 Yushan Rd, Qingdao 266003, Peoples R China

来源：

EUROPEAN JOURNAL OF NUTRITION | 2023年 / 62卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Probiotics; Intestinal barrier; Transcriptomics; Mucin2; Tight junction proteins; EPITHELIAL BARRIER FUNCTION; LACTIC-ACID BACTERIA; REGULATORY T-CELLS; INFLAMMATION; INTERLEUKIN-18; ACTIVATION; PROBIOTICS; COLITIS; DISEASE; IL-10;

D O I：

10.1007/s00394-022-03064-x

中图分类号：

R15 [营养卫生、食品卫生]; TS201 [基础科学];

学科分类号：

100403 ;

摘要：

Purpose Numerous studies have found that probiotics benefit the intestinal barrier. However, the prophylactic effects of probiotics on the intestinal barrier, i.e., if probiotics exert protective effects in healthy individuals to defend them against harmful elements, have seldomly been reported. The present study aimed to investigate the possible mechanisms of potential strains with the function of preventing intestinal barrier damage. Methods This study investigated nine potential probiotic strains using in vitro and in vivo models on their intestinal barrier-protecting properties. Transcriptomic was then employed to decipher the underlying mechanisms of action of the strains. Results The results showed that the strains, to varying degrees, regulated the ratio of interleukin (IL)-10 and IL-12 in peripheral blood mononuclear cells (PBMCs), increased the transepithelial electrical resistance (TEER) values, and decreased Caco-2 cell monolayers permeability. Correspondingly, the strains showed different prophylactic efficacies in protecting mice from dextran sulfate sodium (DSS)-induced intestinal barrier damage. Remarkably, Bifidobacterium bifidum FL-228.1 (FL-228.1) showed the best prophylactic efficacies in protecting mice from DSS-induced intestinal barrier damage. Further research suggested that FL-228.1 exerted its prophylactic effects by enhancing mucin 2 (Muc2) production and Claudin (Cldn)-4 in the colon. Furthermore, the transcriptomic and protein-protein interactions (PPI) analyses indicated that the inhibition of NLRP3 and the activation of PPAR gamma and TLR2 could be involved in protecting the intestinal barrier by FL-228.1. Conclusion Bifidobacterium bifidum FL-228.1 may be developed as a promising probiotic for the prevention of intestinal barrier damage via PPAR gamma/NLRP3/ TLR2 pathways by enhancing Muc2 and Cldn-4.

引用

页码：1267 / 1280

页数：14

共 55 条

[1] Quantitating the cell: turning images into numbers with ImageJ [J].

Arena, Ellen T. ;

Rueden, Curtis T. ;

Hiner, Mark C. ;

Wang, Shulei ;

Yuan, Ming ;

Eliceiri, Kevin W. .

WILEY INTERDISCIPLINARY REVIEWS-DEVELOPMENTAL BIOLOGY, 2017, 6 (02)

[2] Lactic acid bacteria and probiotic organisms induce different cytokine profile and regulatory T cells mechanisms [J].

Ashraf, R. ;

Vasiljevic, T. ;

Day, S. L. ;

Smith, S. C. ;

Donkor, O. N. .

JOURNAL OF FUNCTIONAL FOODS, 2014, 6 :395-409

[3] Toll-like receptor 2 controls mucosal inflammation by regulating epithelial barrier function [J].

Cario, E. ;

Gerken, G. ;

Podolsky, D. K. .

GASTROENTEROLOGY, 2007, 132 (04) :1359-1374

[4] Relative expression of bacterial and host specific genes associated with probiotic survival and viability in the mice gut fed with Lactobacillus plantarum Lp91 [J].

Chandran, Archana ;

Duary, Raj Kumar ;

Grover, Sunita ;

Batish, Virender Kumar .

MICROBIOLOGICAL RESEARCH, 2013, 168 (09) :555-562

[5] In Vitro Characterization of Gut Microbiota-Derived Commensal Strains: Selection of Parabacteroides distasonis Strains Alleviating TNBS-Induced Colitis in Mice [J].

Cuffaro, Bernardo ;

Assohoun, Aka L. W. ;

Boutillier, Denise ;

Sukenikova, Lenka ;

Desramaut, Jeremy ;

Boudebbouze, Samira ;

Salome-Desnoulez, Sophie ;

Hrdy, Jiri ;

Waligora-Dupriet, Anne-Judith ;

Maguin, Emmanuelle ;

Grangette, Corinne .

CELLS, 2020, 9 (09)

[6]

Cui QY, 2022, FOOD FUNCT, V13, P5115, DOI [10.1039/d1fo04219j, 10.1039/D1FO04219J]

[7] New therapies for inflammatory bowel disease: from the bench to the bedside [J].

Danese, Silvio .

GUT, 2012, 61 (06) :918-932

[8] Lactic acid bacteria differ in their ability to induce functional regulatory T cells in humans [J].

de Roock, S. ;

van Elk, M. ;

van Dijk, M. E. A. ;

Timmerman, H. M. ;

Rijkers, G. T. ;

Prakken, B. J. ;

Hoekstra, M. O. ;

de Kleer, I. M. .

CLINICAL AND EXPERIMENTAL ALLERGY, 2010, 40 (01) :103-110

[9] Generation of Tumor-Reactive T Cells by Co-culture of Peripheral Blood Lymphocytes and Tumor Organoids [J].

Dijkstra, Krijn K. ;

Cattaneo, Chiara M. ;

Weeber, Fleur ;

Chalabi, Myriam ;

van de Haar, Joris ;

Fanchi, Lorenzo F. ;

Slagter, Maarten ;

van der Velden, Daphne L. ;

Kaing, Sovann ;

Kelderman, Sander ;

van Rooij, Nienke ;

van Leerdam, Monique E. ;

Depla, Annekatrien ;

Smit, Egbert F. ;

Hartemink, Koen J. ;

de Groot, Rosa ;

Wolkers, Monika C. ;

Sachs, Norman ;

Snaebjornsson, Petur ;

Monkhorst, Kim ;

Haanen, John ;

Clevers, Hans ;

Schumacher, Ton N. ;

Voest, Emile E. .

CELL, 2018, 174 (06) :1586-+

[10] Protective effects of Lacticaseibacillus rhamnosus Hao9 on dextran sulphate sodium-induced ulcerative colitis in mice [J].

Han, Mei ;

Liao, Wenyan ;

Si, Xiaojing ;

Bai, Chen ;

Gai, Zhonghui .

JOURNAL OF APPLIED MICROBIOLOGY, 2022, 133 (03) :2039-2049

← 1 2 3 4 5 6 →