Electron microscopic observation of Mycobacterium avium subsp. paratuberculosis in cattle feces after a novel purification using liquid paraffin

被引：0
作者：
Fujihara, Masatoshi [1 ]
Kondoh, Daisuke [2 ]
Matsuzawa, Shigeru [1 ]
Naito, Ikunori [3 ]
机构：
[1] Hokkaido Kushiro Livestock Hyg Serv Ctr, Kushiro, Hokkaido, Japan
[2] Obihiro Univ Agr & Vet Med, Dept Vet Med, Obihiro, Hokkaido, Japan
[3] Local Independent Adm Agcy Hokkaido Res Org, Shioku, Hokkaido, Japan
来源：
JOURNAL OF MICROBIOLOGICAL METHODS | 2024年 / 217卷
关键词：
Clump; Electron microscopy; Mycobacterium avium subsp. paratuberculosis; TUBERCULOSIS;
D O I：
10.1016/j.mimet.2024.106891
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We developed a novel method for purifying acid-fast bacteria from feces. The method enabled the observation of characteristic clumps of Mycobacterium avium subsp. paratuberculosis (MAP) under electron microscopy by removing contaminants and other bacteria. Further refinement of this method will contribute to efficient and effective MAP detection.
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页数：4
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