Distinctive Biological Properties between Mesenchymal Stem Cell Spheroids and Clumps of Mesenchymal Stem Cells/Extracellular Matrix Complexes in 3D Culture Systems

被引：0

作者：

Yoshino, Mai ^{[1
]}

Kajiya, Mikihito ^{[1
,2
]}

Yoshii, Hiroki ^{[1
]}

Morimoto, Shin ^{[1
]}

Horikoshi, Susumu ^{[1
]}

Tari, Misako ^{[1
]}

Iwata, Tomoyuki ^{[1
]}

Ouhara, Kazuhisa ^{[1
]}

Ando, Toshinori ^{[2
]}

Yoshimoto, Tetsuya ^{[2
]}

Shintani, Tomoaki ^{[2
]}

Mizuno, Noriyoshi ^{[1
]}

机构：

[1] Hiroshima Univ, Grad Sch Biomed & Hlth Sci, Dept Periodontal Med, 1-2-3 Kasumi,Minami ku, Hiroshima 7348553, Japan

[2] Hiroshima Univ Hosp, Dept Innovat & Precis Dent, 1-2-3 Kasumi,Minami Ku, Hiroshima 7348553, Japan

来源：

APPLIED SCIENCES-BASEL | 2023年 / 13卷 / 23期

基金：

日本学术振兴会;

关键词：

MSCs; 2; 3D culture 3; MSC spheroids 4; C-MSCs; 5; cell-cell contact 6; ECM-cell interactions; STROMAL CELLS; CORD BLOOD; DIFFERENTIATION; COMPACTION; REGULATORS; PGE2; MSCS;

D O I：

10.3390/app132312790

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Featured Application Through a comprehensive comparison between the widely-used MSC spheroids and the newly-developed C-MSCs, this study sheds light on the distinct biological properties of each 3D cell culture method, advancing our understanding and potentially paving the way for more effective and versatile applications in MSC-based cell therapy.Abstract Background: Cells typically function and behave within a three-dimensional (3D) environment. Mesenchymal stem cells (MSCs), known for their self-renewal, multi-lineage differentiation capabilities, and paracrine effects, have garnered significant medical interest. MSC spheroid culture is widely adopted to study the biological properties of MSCs in a 3D context. In contrast, we previously developed 3D clumps of MSC/ECM complexes termed C-MSCs. C-MSCs consisted of cells and self-produced ECM proteins, allowing grafting into tissue defects without any artificial scaffolds. This present study aimed to elucidate the fundamental biological distinctions between 3D MSC spheroids and C-MSCs. Methods: MSC spheroids and C-MSCs are generated from human bone-marrow-derived MSCs. The physical properties, histological structures, and gene expression patterns were compared in vitro. Results: Macroscopic and histological examinations revealed that, whereas MSC spheroids are dense cell clusters primarily formed through Cadherin-mediated cell-cell interactions, C-MSCs are cell aggregates anchored by the ECM component COL1, enabling them to form larger structures. Furthermore, transcriptome analysis showed that C-MSCs possess enhanced capacities to produce immunomodulatory and cytoprotective factors, a prominent biological characteristic of MSCs. Conclusion: Recognizing the distinct attributes of each cell aggregate offers insights into the potential evolution of 3D cell culture techniques and possible therapeutic implications.

引用

页数：13

共 40 条

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