In situ-formed hydroxyapatite and poly (lactic-co-glycolic acid) injectable implants as the cargo loading of bioactive substances for bone regeneration

被引:2
作者
Wang, Zongliang [1 ]
Zhang, Ning [2 ]
Liu, Jianguo [3 ]
Xu, Zhiqiang [4 ]
Wang, Liqiang [5 ]
Wang, Yu [1 ]
Wang, Yifan [2 ]
Zhang, Peibiao [1 ]
机构
[1] Chinese Acad Sci, Changchun Inst Appl Chem, Key Lab Polymer Ecomat, Changchun 130022, Peoples R China
[2] Shandong Univ, Dept Foot & Ankle Surg, Hosp 2, Jinan 250033, Peoples R China
[3] First Hosp Jilin Univ, Dept Orthoped, R China, Changchun 130021, Peoples R China
[4] Foshan Hosp Tradit Chinese Med, Dept Rehabil Med, Foshan 528000, Peoples R China
[5] Chinese Peoples Liberat Army Gen Hosp, Med Ctr 3, Dept Ophthalmol, Beijing 100853, Peoples R China
基金
中国国家自然科学基金;
关键词
GELATIN MICROSPHERES; SCAFFOLDS; DELIVERY; TISSUE; SYSTEMS; CELLS; VITRO; VIVO; NMP;
D O I
10.1039/d3nj01195j
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In this study, we developed an injectable system of hydroxyapatite and poly (lactic-co-glycolic acid) (HA/PLGA) with an in situ-solidification property and appropriate initial mechanical strength through the phase separation of a water-miscible solvent. Gelatin microspheres (GMs) were fabricated and utilized as porogen and a potential cargo loading of the biological substance of bone morphogenetic protein 2 (BMP-2). The injectability, compression strength, and microstructure of the injectable implants were systematically characterized by screening the molecule weight (M-w) and concentration of PLGA solution and the proportion of PLGA, HA, and GMs. It was noticed that a higher M-w and concentration of PLGA matrix and a moderate content of HA and GMs resulted in a satisfactory injectable force and mechanical property. In vivo evaluation demonstrated that the injectable materials could significantly repair skull defects, especially via efficient loading with BMP-2, which implied these could serve as a potential vehicle of biological substances for bone tissue regeneration. Although there was acute cytotoxicity after injection, no damage was observed for the main organs by these injectable materials. In all, the GM-incorporated injectable implants present a promising aspect for realizing innovative minimally invasive treatment for clinical orthopedics.
引用
收藏
页码:11822 / 11831
页数:10
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