Fabrication of a simple 3D-printed microfluidic device with embedded electrochemiluminescence detection for rapid determination of sibutramine in dietary supplements

被引：5

作者：

Praoboon, Nisachon ^{[1
]}

Tangkuaram, Tanin ^{[1
]}

Kruefu, Viruntachar ^{[2
]}

Pookmanee, Pusit ^{[1
]}

Phaisansuthichol, Sirirat ^{[1
]}

Kuimalee, Surasak ^{[3
]}

Laorodphan, Nattapol ^{[3
]}

Satienperakul, Sakchai ^{[1
]}

机构：

[1] Maejo Univ, Fac Sci, Dept Chem, Chiang Mai 50290, Thailand

[2] Maejo Univ, Fac Sci, Dept Phys, Chiang Mai 50290, Thailand

[3] Maejo Univ, Fac Sci, Dept Ind Chem Innovat, Chiang Mai 50290, Thailand

来源：

MICROCHIMICA ACTA | 2023年 / 190卷 / 04期

关键词：

Embedded electrochemiluminescence; 3D-printing technology; Sibutramine; Dietary supplements; GOLD NANOPARTICLES; BIOSENSOR;

D O I：

10.1007/s00604-023-05725-y

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The design and fabrication of a simple 3D-printed platform with embedded electrochemiluminescence (ECL) detection for sibutramine determination is described. The microfluidic platform was fabricated by the fused deposition 3D-printing technique with polylactic acid filament, facilitated by computer-aided design (CAD). A three-electrode system was integrated into the device using graphene carbon paste as a working electrode, Ag/AgCl wire as a reference, and a graphite rod as a counter electrode. A further modification was carried out by applying bimetallic Au-Pt nanoparticle-supported multi-walled carbon nanotubes (MWCNT-Au-Pt) on the working electrode surface to enhance the electrocatalytic performance by exploiting the unique properties of nanomaterials. The analytical feasibility of the CAD-ECL sensor was tested through its application for the determination of sibutramine in dietary supplements. Under the optimized conditions, based on the enhancing effect of luminol emission, the device exhibited a linear calibration curve of the logarithmic sibutramine concentration versus ECL intensity in the range 5 x 10(-3) to 1 ng mL(-1). The limit of detection was 3 pg mL(-1) with a relative standard deviation of 1.7% (n = 15). The 3D-printed prototype can be successfully applied to a small-scale analysis in a simple and cost-effective approach.

引用

页数：12