Evaluation of Probiotic Efficacy of Bacillus subtilis RODK28110C3 Against Pathogenic Aeromonas hydrophila and Edwardsiella tarda Using In Vitro Studies and In Vivo Gnotobiotic Zebrafish Gut Model System

被引：6

作者：

Nayak, Ashwath ^{[1
]}

Harshitha, Mave ^{[1
]}

Dubey, Saurabh ^{[2
]}

Munang'andu, Hetron Mweemba ^{[3
]}

Chakraborty, Anirban ^{[4
]}

Karunasagar, Indrani ^{[5
]}

Maiti, Biswajit ^{[1
]}

机构：

[1] Nitte Deemed Univ, Nitte Univ Ctr Sci Educ & Res, Div Infect Dis, Paneer Campus, Mangaluru 575018, India

[2] Norwegian Univ Life Sci, Fac Vet Med, Dept Prod Anim Clin Sci, Sect Expt Biomed, As, Norway

[3] Nord Univ, Dept Biosci & Aquaculture, PB 1490, N-8049 Bodo, Norway

[4] Nitte Deemed Univ, Nitte Univ Ctr Sci Educ & Res, Div Mol Genet & Canc, Mangaluru 575018, India

[5] Nitte Deemed Univ, DST Technol Enabling Ctr, Paneer Campus, Mangaluru 575018, India

来源：

PROBIOTICS AND ANTIMICROBIAL PROTEINS | 2024年 / 16卷 / 05期

关键词：

Bacillus subtilis; Competitive inhibition; Fish pathogen; Probiotic; Zebrafish; GROWTH-PERFORMANCE; IMMUNE-RESPONSE;

D O I：

10.1007/s12602-023-10127-w

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The indiscriminate use of antibiotics in aquaculture has led to the emergence of resistance; hence, eco-friendly, host-specific alternatives to mitigate bacterial infections have become imminent. In this study, bacteria that could possibly serve as probiotics were isolated and evaluated for their efficacy with in vitro experiments and in vivo zebrafish gut model. One isolate from each of the 23 rohu fish (Labeo rohita) was shortlisted after preliminary screening of several isolates and tested for their ability to inhibit two important warm water bacterial fish pathogens, Aeromonas hydrophila, and Edwardsiella tarda. An isolate (RODK28110C3) that showed broad-spectrum inhibitory activity against a battery of different isolates of the two fish pathogens included in this study and maintained in our repository was selected for further characterization. The culture was identified phenotypically as Bacillus subtilis and confirmed by 16S rDNA sequencing. The isolate was able to hydrolyze fish feed constituents that include starch, protein, and cellulose. Further in vitro tests ensured that the potential isolate with probiotic attributes could tolerate different gut conditions, which included a range of pH, salinity, and varying concentrations of bile salt. Exposure of 4 days post fertilization zebrafish embryos to the RFP-tagged isolate confirmed the colonization of B. subtilis in the gut of the zebrafish embryo, which is an important attribute of a probiotic. The isolate was able to inhibit both A. hydrophila and E. tarda in gnotobiotic zebrafish embryo in triplicate. The study demonstrates the probiotic characteristics of the B. subtilis isolated from L. rohita and its ability to inhibit A. hydrophila and E. tarda using in vitro conditions and in the zebrafish gut and could serve as an effective alternative to antibiotics in aquaculture.

引用

页码：1623 / 1637

页数：15

共 54 条

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