Radix Pueraria lobata polysaccharide relieved DSS-induced ulcerative colitis through modulating PI3K signaling

被引：4

作者：

Zhang, Zhuang ^{[1
]}

Cui, Yushun ^{[1
]}

Ouyang, Hui ^{[1
]}

Zhu, Weifeng ^{[2
]}

Feng, Yulin ^{[1
]}

Yao, Min ^{[3
]}

Yang, Shilin ^{[1
]}

机构：

[1] Jiangxi Univ Chinese Med, Natl Engn Res Ctr Mfg Technol Solid Preparat Tradi, 56 Yangming Rd, Nanchang 330006, Peoples R China

[2] Jiangxi Univ Chinese Med, Key Lab Modern Preparat Chinese Med, 818 Yunwan Rd, Nanchang 330002, Peoples R China

[3] Jiangxi Inst Drug Inspection & Testing, State Drug Adm Key Lab Qual Evaluat Proprietary Ch, Nanchang 330029, Peoples R China

来源：

JOURNAL OF FUNCTIONAL FOODS | 2023年 / 104卷

关键词：

Radix Puerariae lobata polysaccharide; Ulcerative colitis; Gut microbiota; Metabonomics; Knockdown; PI3K; INFLAMMATORY-BOWEL-DISEASE; CACO-2; CELL-LINE; STRUCTURAL-CHARACTERIZATION; INTESTINAL BARRIER; GUT; MICROBIOTA; APOPTOSIS; PATHWAY; SODIUM; MODEL;

D O I：

10.1016/j.jff.2023.105514

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Polysaccharides are the essential active constituents of Radix Pueraria lobata. Numerous studies have shown that polysaccharides can play important roles in ulcerative colitis (UC). This study aimed to determine the protective effects of Radix Pueraria lobata polysaccharide (PPL) against UC. The pharmacological effects of PPL on the dextran sulfate sodium (DSS)-induced UC mice, changes in gut microbiota and metabolism, and DSS-induced Caco-2 cell injury were assessed in this study. Both the PPL inhibited the DSS-induced inflammation both in vivo and in vitro. Mechanistically, PPL inhibited inflammation by decreasing the activation of p-PI3K. Knocking down the PI3K gene resulted in abolishing the inhibitory effects of PPL on mouses and Caco-2 cells. The study concluded that the effects of PPL were completely dependent on PI3K.

引用

页数：11

共 50 条

[1] Regulation of TNF-α and NF-κB activation through the JAK/STAT signaling pathway downstream of histamine 4 receptor in a rat model of LPS-induced joint inflammation
Ahmad, Sheikh Fayaz
Ansari, Mushtaq Ahmad
Zoheir, Khairy M. A.
Bakheet, Saleh A.
Korashy, Hesham M.
Nadeem, Ahmed
Ashour, Abdelkader E.
Attia, Sabry M.
[J]. IMMUNOBIOLOGY, 2015, 220 (07) : 889 - 898
[2] Araki Y, 2006, ONCOL REP, V16, P1357
[3] Treatment of IBD: Where We Are and Where We Are Going
Bernstein, Charles N.
[J]. AMERICAN JOURNAL OF GASTROENTEROLOGY, 2015, 110 (01) : 114 - 126
[4] Fat of the Gut: Epithelial Phospholipids in Inflammatory Bowel Diseases
Boldyreva, Lidiya V.
Morozova, Maryana V.
Saydakova, Snezhanna S.
Kozhevnikova, Elena N.
[J]. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2021, 22 (21)
[5] Inflammatory bowel disease and immunonutrition: novel therapeutic approaches through modulation of diet and the gut microbiome
Celiberto, Larissa S.
Graef, Franziska A.
Healey, Genelle R.
Bosman, Else S.
Jacobson, Kevan
Sly, Laura M.
Vallance, Bruce A.
[J]. IMMUNOLOGY, 2018, 155 (01) : 36 - 52
[6] Chang KW, 2015, FOOD FUNCT, V6, P3334, DOI [10.1039/c5fo00513b, 10.1039/C5FO00513B]
[7] Effects of polysaccharide from Pueraria lobata on gut microbiota in mice
Chen, Rong
Liu, Bo
Wang, Xiaoyang
Chen, Kai
Zhang, Keyu
Zhang, Lifang
Fei, Chenzhong
Wang, Chunmei
Liu, Yingchun
Xue, Feiqun
Gu, Feng
Wang, Mi
[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 158 : 740 - 749
[8] Cui L., 2021, INT J BIOL MACROMOL
[9] Structural characterization and immunomodulatory activity of a novel polysaccharide from Pueraria lobata (Willd.) Ohwi root
Dong, Zhou
Zhang, Mengmeng
Li, Huixian
Zhan, Qiping
Lai, Furao
Wu, Hui
[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 154 : 1556 - 1564
[10] Polysaccharides from Atractylodes macrocephala Koidz. Ameliorate ulcerative colitis via extensive modification of gut microbiota and host metabolism
Feng, Wuwen
Liu, Juan
Tan, Yuzhu
Ao, Hui
Wang, Jing
Peng, Cheng
[J]. FOOD RESEARCH INTERNATIONAL, 2020, 138

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