Outer Membrane Vesicles Associated With the Delivery of Amoxicillin Resistance in Glaesserella parasuis

被引：3

作者：

An, Jiahui ^{[1
]}

Zhang, Chao ^{[1
]}

Li, Yufeng ^{[1
,2
]}

机构：

[1] Nanjing Agr Univ, Coll Vet Med, Key Lab Bacteriol, Minist Agr, Nanjing, Peoples R China

[2] Nanjing Agr Univ, Coll Vet Med, Key Lab Bacteriol, Minist Agr, Nanjing 210095, Peoples R China

来源：

MICROBIAL DRUG RESISTANCE | 2023年 / 29卷 / 06期

关键词：

Glaesserella parasuis; outer membrane vesicles; beta-lactamase; drug resistance; BETA-LACTAM RESISTANCE; HAEMOPHILUS-PARASUIS; BIOGENESIS; INFLUENZAE;

D O I：

10.1089/mdr.2022.0184

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

Glasser's disease is caused by Glaesserella parasuis, a common bacterium in the upper respiratory tract of pigs. Antibiotics are widely used to control this disease. A G. parasuis isolate with amoxicillin (AMX) resistance was identified in our previous study. Outer membrane vesicles (OMVs) are naturally released by G. parasuis and contain many compounds. To reveal the underlying mechanisms associated with AMX resistance delivery, OMVs from G. parasuis were successfully isolated and identified by transmission electron microscopy. In particular, we found that beta-lactamase existed in OMVs using label-free analysis, and further verified that OMVs carry beta-lactamase by Western blotting. The minimal inhibitory concentration and growth rate were determined to evaluate the beta-lactamase activity in G. parasuis OMVs. Moreover, the effect of different concentrations of OMVs from aHPS7 on the growth rate of AMX sensitive strains was examined. Our results further confirmed that OMVs isolated from aHPS7 contain beta-lactamase, which can prevent AMX-susceptible strains from killing by hydrolyzing AMX. Our results first showed that OMVs of G. parasuis play an important role in the spread of antibiotic resistance, which seriously hampered the prevention of this disease by the delivery of OMVs in different strains.

引用

页码：263 / 270

页数：8

共 34 条

[11] The Importance of Porins and β-Lactamase in Outer Membrane Vesicles on the Hydrolysis of β-Lactam Antibiotics [J].

Kim, Si Won ;

Lee, Jung Seok ;

Park, Seong Bin ;

Lee, Ae Rin ;

Jung, Jae Wook ;

Chun, Jin Hong ;

Lazarte, Jassy Mary S. ;

Kim, Jaesung ;

Seo, Jong-Su ;

Kim, Jong-Hwan ;

Song, Jong-Wook ;

Ha, Min Woo ;

Thompson, Kim D. ;

Lee, Chang-Ro ;

Jung, Myunghwan ;

Jung, Tae Sung .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (08)

[12] Biogenesis and multifaceted roles of outer membrane vesicles from Gram-negative bacteria [J].

Kulkarni, Heramb M. ;

Jagannadham, Medicharla V. .

MICROBIOLOGY-SGM, 2014, 160 :2109-2121

[13] Biological Functions and Biogenesis of Secreted Bacterial Outer Membrane Vesicles [J].

Kulp, Adam ;

Kuehn, Meta J. .

ANNUAL REVIEW OF MICROBIOLOGY, VOL 64, 2010, 2010, 64 :163-184

[14] Antimicrobial susceptibility patterns of Haemophilus parasuis from pigs in the United Kingdom and Spain [J].

Martin de la Fuente, A. J. ;

Tucker, A. W. ;

Navas, J. ;

Blanco, M. ;

Morris, S. J. ;

Gutierrez-Martin, C. B. .

VETERINARY MICROBIOLOGY, 2007, 120 (1-2) :184-191

[15] On the Offensive: the Role of Outer Membrane Vesicles in the Successful Dissemination of New Delhi Metallo-β-lactamase (NDM-1) [J].

Martinez, Melina M. B. ;

Bonomo, Robert A. ;

Vila, Alejandro J. ;

Maffia, Paulo C. ;

Gonzalez, Lisandro J. .

MBIO, 2021, 12 (05)

[16] Characterization and Vaccine Potential of Outer Membrane Vesicles Produced by Haemophilus parasuis [J].

McCaig, William D. ;

Loving, Crystal L. ;

Hughes, Holly R. ;

Brockmeier, Susan L. .

PLOS ONE, 2016, 11 (03)

[17] Novel blaROB-1-Bearing Plasmid Conferring Resistance to β-Lactams in Haemophilus parasuis Isolates from Healthy Weaning Pigs [J].

Moleres, Javier ;

Santos-Lopez, Alfonso ;

Lazaro, Isidro ;

Labairu, Javier ;

Prat, Cristina ;

Ardanuy, Carmen ;

Gonzalez-Zorn, Bruno ;

Aragon, Virginia ;

Garmendia, Junkal .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2015, 81 (09) :3255-3267

[18] OPTIMALIZATION OF THE DETECTION OF NAD DEPENDENT PASTEURELLACEAE FROM THE RESPIRATORY-TRACT OF SLAUGHTERHOUSE PIGS [J].

MOLLER, K ;

ANDERSEN, LV ;

CHRISTENSEN, G ;

KILIAN, M .

VETERINARY MICROBIOLOGY, 1993, 36 (3-4) :261-271

[19] Haemophilus parasuis:: new trends on diagnosis, epidemiology and control [J].

Oliveira, S ;

Pijoan, C .

VETERINARY MICROBIOLOGY, 2004, 99 (01) :1-12

[20] Structure and immunological action of the human pathogen Moraxella catarrhalis IgD-binding protein [J].

Riesbeck, Kristian ;

Nordstrom, Therese .

CRITICAL REVIEWS IN IMMUNOLOGY, 2006, 26 (04) :353-376

← 1 2 3 4 →