Evaluation of porous bacterial cellulose produced from foam templating with different additives and its application in 3D cell culture

被引：17
作者：
Lin, Shin-Ping [1 ,2 ,3 ]
Singajaya, Stephanie [4 ]
Lo, Tsui-Yun [5 ]
Santoso, Shella Permatasari [6 ,7 ]
Hsu, Hsien-Yi [8 ,9 ]
Cheng, Kuan-Chen [4 ,10 ,11 ,12 ]
机构：
[1] Taipei Med Univ, Sch Food Safety, 250 Wu Hsing St, Taipei 11031, Taiwan
[2] Taipei Med Univ, TMU Res Ctr Digest Med, 250 Wu Hsing St, Taipei 11031, Taiwan
[3] Taipei Med Univ, Res Ctr Biomed Device, 250 Wu Hsing St, Taipei 11031, Taiwan
[4] Natl Taiwan Univ, Inst Biotechnol, 1 Roosevelt Rd,Sec 4, Taipei 10617, Taiwan
[5] Taipei Med Univ, Sch Med, Dept Biochem & Mol Cell Biol, 250 Wu Hsing St, Taipei 11031, Taiwan
[6] Widya Mandala Surabaya Catholic Univ, Dept Chem Engn, Kalijudan 37, Surabaya 60114, Indonesia
[7] Collaborat Res Ctr Sustainable & Zero Waste Ind, Kalijudan 37, Surabaya 60114, East Java, Indonesia
[8] City Univ Hong Kong, Sch Energy & Environm, Dept Mat Sci & Engn, Kowloon Tong, Hong Kong 518057, Peoples R China
[9] City Univ Hong Kong, Shenzhen Res Inst, Hong Kong 518057, Peoples R China
[10] Natl Taiwan Univ, Grad Inst Food Sci & Technol, 1 Roosevelt Rd,Sec 4, Taipei 10617, Taiwan
[11] China Med Univ, China Med Univ Hosp, Dept Med Res, 91 Hsueh Shih Rd, Taichung 40402, Taiwan
[12] Asia Univ, Dept Optometry, 500 Lioufeng Rd, Taichung 41354, Taiwan
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2023年 / 234卷
关键词：
Bacterial cellulose; Tissue scaffold; 3D cell culture; Foam templating; SCAFFOLDS;
D O I：
10.1016/j.ijbiomac.2023.123680
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Bacterial cellulose (BC) is used in biomedical applications due to its unique material properties such as me-chanical strength with a high water-absorbing capacity and biocompatibility. Nevertheless, native BC lacks porosity control which is crucial for regenerative medicine. Hence, developing a simple technique to change the pore sizes of BC has become an important issue. This study combined current foaming BC (FBC) production with incorporation of different additives (avicel, carboxymethylcellulose, and chitosan) to form novel porous additive-altered FBC. Results demonstrated that the FBC samples provided greater reswelling rates (91.57 % -93.67 %) compared to BC samples (44.52 % -67.5 %). Moreover, the FBC samples also showed excellent cell adhesion and proliferation abilities for NIH-3T3 cells. Lastly, FBC allowed cells to penetrate to deep layers for cell adhesion due to its porous structure, providing a competitive scaffold for 3D cell culture in tissue engineering.
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页数：9
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