Ginsenoside Rk3 ameliorates Aβ-induced neurotoxicity in APP/PS1 model mice via AMPK signaling pathway

被引：26

作者：

She, Lingyu ^{[1
,2
,3
]}

Xiong, Li ^{[2
,3
]}

Li, Liwei ^{[3
]}

Zhang, Jing ^{[2
]}

Sun, Jinfeng ^{[1
,3
]}

Wu, Haibin ^{[3
]}

Ren, Juan ^{[4
]}

Wang, Wei ^{[2
]}

Zhao, Xia ^{[2
,3
]}

Liang, Guang ^{[1
,2
,3
]}

机构：

[1] Yanbian Univ, Key Lab Nat Med Changbai Mt, Minist Educ, Yanji 133002, Jilin, Peoples R China

[2] Affiliated Yongkang First Peoples Hosp, Hangzhou Med Coll, Yongkang 321399, Zhejiang, Peoples R China

[3] Hangzhou Med Coll, Sch Pharmaceut Sci, Hangzhou 311399, Zhejiang, Peoples R China

[4] Hangzhou Med Coll, Sch Lab Med & Bioengineer, Hangzhou 311399, Zhejiang, Peoples R China

来源：

BIOMEDICINE & PHARMACOTHERAPY | 2023年 / 158卷

关键词：

Alzheimer?s disease; Ginsenoside Rk3; Oxidative stress; APP; PS1; AMPK signaling pathway; AMYLOID PRECURSOR PROTEIN; PROMOTES NONAMYLOIDGENIC CLEAVAGE; PANAX-GINSENG; MECHANISMS; RG1; PHOSPHORYLATION; DEPOSITION; RD;

D O I：

10.1016/j.biopha.2022.114192

中图分类号：

R-3 [医学研究方法]; R3 [基础医学];

学科分类号：

1001 ;

摘要：

Alzheimer's disease (AD) has become a major public health problem affecting the elderly population, and there is currently no effective treatment. Although the pathogenesis of AD is unclear, neurotoxicity induced by oxidative stress plays an important role in the progression of AD. Ginseng, the root and rhizome of Panax ginseng C. A. Meyer, is used not only as an herbal medicine but also as a functional food to support bodily functions. Gin-senoside Rk3 (Rk3), the main bioactive component in ginseng, has a strong antioxidant effect and has not been reported in AD. In this study, we showed that Rk3 improved neuronal apoptosis, decreased intracellular reactive oxygen species (ROS) production and restored mitochondrial membrane potential in PC12 and primary neuronal cells. In vivo, we found that Rk3 improved spatial learning and memory deficit in precursor protein (APP)/ presenilin 1 (PS1) double transgenic mouse model of AD. Additionally, Rk3 increases glutathione reductase (GSH) and superoxide dismutase (SOD) levels while inhibits malondialdehyde (MDA) production, apoptosis and activation of glial cells in APP/PS1 mice. Mechanistically, we found that the protective effect of Rk3 is in cor-relation with the activation of AMPK/Nrf2 signaling pathway. In conclusion, the findings of this study provide support for Rk3 as a new strategy for the treatment of AD.

引用

页数：16