Coumarin-Based Fluorescence Probe for Differentiated Detection of Biothiols and Its Bioimaging in Cells

被引：9
作者：
Du, Wei [1 ]
Gong, Xiu-Lin [1 ]
Tian, Yang [1 ]
Zhu, Xi [2 ]
Peng, Yu [1 ]
Wang, Ya-Wen [1 ]
机构：
[1] Southwest Jiaotong Univ, Sch Life Sci & Engn, Sch Chem, Chengdu 610031, Peoples R China
[2] Southwest Jiaotong Univ, Peoples Hosp Chengdu 3, Dept Neurol, Affiliated Hosp, Chengdu 610031, Peoples R China
来源：
BIOSENSORS-BASEL | 2023年 / 13卷 / 04期
基金：
中国国家自然科学基金;
关键词：
biothiols; fluorescence; coumarin derivative; LIQUID-CHROMATOGRAPHY; AMPEROMETRIC DETECTION; SELECTIVE DETECTION; LIVING CELLS; CYSTEINE; GLUTATHIONE; HOMOCYSTEINE; THIOLS; CYS; GSH;
D O I：
10.3390/bios13040447
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
In this work, a coumarin derivative, SWJT-14, was synthesized as a fluorescence probe to distinguish cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) in aqueous solutions. The detection limit of Cys, Hcy and GSH for the probe was 0.02 mu M, 0.42 mu M and 0.92 mu M, respectively, which was lower than biothiols in cells. The probe reacted with biothiols to generate different products with different conjugated structures. Additionally, it could distinguish Cys, Hcy and GSH using fluorescence and UV-Vis spectra. The detection mechanism was confirmed by MS. SWJT-14 was successfully used in cellular experiments and detected both endogenous and exogenous biothiols.
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页数：10
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