Microfluidics: the propellant of CRISPR-based nucleic acid detection

被引：48

作者：

Chen, Yanju ^{[1
]}

Qian, Siwenjie ^{[1
]}

Yu, Xiaoping ^{[3
]}

Wu, Jian ^{[1
,4
]}

Xu, Junfeng ^{[2
]}

机构：

[1] Zhejiang Univ, Coll Biosyst Engn & Food Sci, Hangzhou 310058, Peoples R China

[2] Zhejiang Acad Agr Sci, Key Lab Traceabil Agr Genet Modified Organisms, Minist Agr & Rural Affairs, Hangzhou 310021, Peoples R China

[3] China Jiliang Univ, Coll Life Sci, Hangzhou 310018, Peoples R China

[4] ZJU Hangzhou Global Sci & Technol Innovat Ctr, 733 Jianshe 3rd Rd, Hangzhou 311200, Peoples R China

来源：

TRENDS IN BIOTECHNOLOGY | 2023年 / 41卷 / 04期

关键词：

SYSTEMS; CLASSIFICATION; AMPLIFICATION; DIAGNOSTICS; UNIVERSAL; CAS12A; ASSAY;

D O I：

10.1016/j.tibtech.2022.07.015

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Since the discovery of collateral cleavage activity, clustered regularly interspaced short palindromic repeats (CRISPR)/Cas systems have become the new generation of nucleic acid detection tools. However, their widespread application remains limited. A pre-amplification step is required to improve the sensitivity of CRISPR systems, complicating the operating procedure and limiting quantitative precision. In addition, nonspecific collateral cleavage activity makes it difficult to realize multiplex detection in a one-pot CRISPR reaction with a single Cas protein. Microfluidics, which can transfer nucleic acid analysis process to a chip, has the advantages of miniaturization, integration, and automation. Microfluidics coupled with CRISPR systems improves the detection ability of CRISPR, enabling fast, high-throughput, integrated, multiplex, and digital detection, which results in the further popularization of CRISPR for a range of scenarios.

引用

页码：557 / 574

页数：18

共 82 条

[1] C2c2 is a single-component programmable RNA-guided RNA-targeting CRISPR effector [J].

Abudayyeh, Omar O. ;

Gootenberg, Jonathan S. ;

Konermann, Silvana ;

Joung, Julia ;

Slaymaker, Ian M. ;

Cox, David B. T. ;

Shmakov, Sergey ;

Makarova, Kira S. ;

Semenova, Ekaterina ;

Minakhin, Leonid ;

Severinov, Konstantin ;

Regev, Aviv ;

Lander, Eric S. ;

Koonin, Eugene V. ;

Zhang, Feng .

SCIENCE, 2016, 353 (6299)

[2] Massively multiplexed nucleic acid detection with Cas13 [J].

Ackerman, Cheri M. ;

Myhrvold, Cameron ;

Thakku, Sri Gowtham ;

Freije, Catherine A. ;

Metsky, Hayden C. ;

Yang, David K. ;

Ye, Simon H. ;

Boehm, Chloe K. ;

Kosoko-Thoroddsen, Tinna-Solveig F. ;

Kehe, Jared ;

Nguyen, Tien G. ;

Carter, Amber ;

Kulesa, Anthony ;

Barnes, John R. ;

Dugan, Vivien G. ;

Hung, Deborah T. ;

Blainey, Paul C. ;

Sabeti, Pardis C. .

NATURE, 2020, 582 (7811) :277-+

[3] Deployable CRISPR-Cas13a diagnostic tools to detect and report Ebola and Lassa virus cases in real-time [J].

Barnes, Kayla G. ;

Lachenauer, Anna E. ;

Nitido, Adam ;

Siddiqui, Sameed ;

Gross, Robin ;

Beitzel, Brett ;

Siddle, Katherine J. ;

Freije, Catherine A. ;

Dighero-Kemp, Bonnie ;

Mehta, Samar B. ;

Carter, Amber ;

Uwanibe, Jessica ;

Ajogbasile, Fehintola ;

Olumade, Testimony ;

Odia, Ikponmwosa ;

Sandi, John Demby ;

Momoh, Mambu ;

Metsky, Hayden C. ;

Boehm, Chloe K. ;

Lin, Aaron E. ;

Kemball, Molly ;

Park, Daniel J. ;

Branco, Luis ;

Boisen, Matt ;

Sullivan, Brian ;

Amare, Mihret F. ;

Tiamiyu, Abdulwasiu B. ;

Parker, Zahra F. ;

Iroezindu, Michael ;

Grant, Donald S. ;

Modjarrad, Kayvon ;

Myhrvold, Cameron ;

Garry, Robert F. ;

Palacios, Gustavo ;

Hensley, Lisa E. ;

Schaffner, Stephen F. ;

Happi, Christian T. ;

Colubri, Andres ;

Sabeti, Pardis C. .

NATURE COMMUNICATIONS, 2020, 11 (01)

[4] CRISPR-Cas12-based detection of SARS-CoV-2 [J].

Broughton, James P. ;

Deng, Xianding ;

Yu, Guixia ;

Fasching, Clare L. ;

Servellita, Venice ;

Singh, Jasmeet ;

Miao, Xin ;

Streithorst, Jessica A. ;

Granados, Andrea ;

Sotomayor-Gonzalez, Alicia ;

Zorn, Kelsey ;

Gopez, Allan ;

Hsu, Elaine ;

Gu, Wei ;

Miller, Steve ;

Pan, Chao-Yang ;

Guevara, Hugo ;

Wadford, Debra A. ;

Chen, Janice S. ;

Chiu, Charles Y. .

NATURE BIOTECHNOLOGY, 2020, 38 (07) :870-+

[5] CRISPR-powered electrochemical microfluidic multiplexed biosensor for target amplification-free miRNA diagnostics [J].

Bruch, Richard ;

Johnston, Midori ;

Kling, Andre ;

Mattmueller, Thorsten ;

Baaske, Julia ;

Partel, Stefan ;

Madlener, Sibylle ;

Weber, Wilfried ;

Urban, Gerald A. ;

Dincer, Can .

BIOSENSORS & BIOELECTRONICS, 2021, 177

[6] CRISPR/Cas13a-Powered Electrochemical Microfluidic Biosensor for Nucleic Acid Amplification-Free miRNA Diagnostics [J].

Bruch, Richard ;

Baaske, Julia ;

Chatelle, Claire ;

Meirich, Mailin ;

Madlener, Sibylle ;

Weber, Wilfried ;

Dincer, Can ;

Urban, Gerald Anton .

ADVANCED MATERIALS, 2019, 31 (51)

[7] Point-of-care CRISPR-Cas-assisted SARS-CoV-2 detection in an automated and portable droplet magnetofluidic device [J].

Chen, Fan-En ;

Lee, Pei-Wei ;

Trick, Alexander Y. ;

Park, Joon Soo ;

Chen, Liben ;

Shah, Kushagra ;

Mostafa, Heba ;

Carroll, Karen C. ;

Hsieh, Kuangwen ;

Wang, Tza-Huei .

BIOSENSORS & BIOELECTRONICS, 2021, 190

[8] CRISPR-Cas12a target binding unleashes indiscriminate single-stranded DNase activity [J].

Chen, Janice S. ;

Ma, Enbo ;

Harrington, Lucas B. ;

Da Costa, Maria ;

Tian, Xinran ;

Palefsky, Joel M. ;

Doudna, Jennifer A. .

SCIENCE, 2018, 360 (6387) :436-+

[9] CRISPR/Cas12a-Based Versatile Method for Checking Quantitative Polymerase Chain Reaction Samples with Cycles of Threshold Values in the Gray Zone [J].

Chen, Yanju ;

Wu, Shuyue ;

Wu, Hui ;

Cheng, Peng ;

Wang, Xiaofu ;

Qian, Siwenjie ;

Zhang, Mengyao ;

Xu, Junfeng ;

Ji, Feng ;

Wu, Jian .

ACS SENSORS, 2021, 6 (05) :1963-1970

[10] Contamination-free visual detection of SARS-CoV-2 with CRISPR/Cas12a: A promising method in the point-of-care detection [J].

Chen, Yanju ;

Shi, Ya ;

Chen, Yin ;

Yang, Zhangnv ;

Wu, Hui ;

Zhou, Zhihui ;

Li, Jue ;

Ping, Jianfeng ;

He, Luping ;

Shen, Hong ;

Chen, Zhengxin ;

Wu, Jian ;

Yu, Yunsong ;

Zhang, Yanjun ;

Chen, Huan .

BIOSENSORS & BIOELECTRONICS, 2020, 169

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