Methicillin-Susceptible Staphylococcus aureus Biofilm Formation on Vascular Grafts: an In Vitro Study

被引：8

作者：

Tello-Diaz, Cristina ^{[1
,2
]}

Palau, Marta ^{[3
,4
]}

Munoz, Estela ^{[3
]}

Gomis, Xavier ^{[3
,4
]}

Gavalda, Joan ^{[3
,4
]}

Fernandez-Hidalgo, Nuria ^{[4
,5
,6
]}

Bellmunt-Montoya, Sergi ^{[2
,3
,4
,7
]}

机构：

[1] Hosp Santa Creu & Sant Pau, Inst Biomed Res II B St Pau, Dept Vasc & Endovascular Surg, CIBER CV, Barcelona, Spain

[2] Univ Autonoma Barcelona UAB, Dept Cirurgia & Ciencies Morfolog, Barcelona, Spain

[3] Hosp Univ Vall Hebron, Vall Hebron Res Inst VHIR, Infect Dis Dept, Antimicrobial Resistance Lab, Barcelona, Spain

[4] CIBER Enfermedades Infecciosas CIBERINFEC, Inst Salud Carlos III ISCIII, Madrid, Spain

[5] Univ Autonoma Barcelona, Hosp Univ Vall Hebron, Infect Dis Dept, Barcelona, Spain

[6] Red Espanola Invest Patol Infecciosa REIPI RD16-0, Inst Salud Carlos III, Madrid, Spain

[7] Hosp Univ Vall Hebron, Dept Angiol Vasc & Endovasc Surg, Barcelona, Spain

来源：

MICROBIOLOGY SPECTRUM | 2023年 / 11卷 / 02期

关键词：

vascular graft; infection; biofilm; Staphylococcus aureus; in vitro; prosthesis infections; SILVER-COATED POLYESTER; OMNIFLOW II; DACRON GRAFT; INFECTION; EFFICACY; POLYTETRAFLUOROETHYLENE; RECONSTRUCTION; PROSTHESES; TRICLOSAN; RESISTANT;

D O I：

10.1128/spectrum.03931-22

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The aim of this study was to quantify in vitro biofilm formation by methicillin-susceptible Staphylococcus aureus (MSSA) on the surfaces of different types of commonly used vascular grafts. We performed an in vitro study with two clinical strains of MSSA (MSSA2 and MSSA6) and nine vascular grafts: Dacron (Hemagard), Dacron-heparin (Intergard heparin), Dacron-silver (Intergard Silver), Dacron-silver-triclosan (Intergard Synergy), Dacron-gelatin (Gelsoft Plus), Dacron plus polytetrafluoroethylene (Fusion), polytetrafluoroethylene (Propaten; Gore), Omniflow II, and bovine pericardium (XenoSure). Biofilm formation was induced in two phases: an initial 90-minute adherence phase and a 24-hour growth phase. Quantitative cultures were performed, and the results were expressed as log(10) CFU per milliliter. The Dacron-silver-triclosan graft and Omniflow II were associated with the least biofilm formation by both MSSA2 and MSSA6. MSSA2 did not form a biofilm on the Dacron-silver-triclosan graft (0 CFU/mL), and the mean count on the Omniflow II graft was 3.89 CFU/mL (standard deviation [SD] 2.10). The mean count for the other grafts was 7.01 CFU/mL (SD 0.82). MSSA6 formed a biofilm on both grafts, with 2.42 CFU/mL (SD 2.44) on the Dacron-silver-triclosan graft and 3.62 CFU/mL (SD 2.21) on the Omniflow II. The mean biofilm growth on the remaining grafts was 7.33 CFU/mL (SD 0.28). The differences in biofilm formation on the Dacron-silver-triclosan and Omniflow II grafts compared to the other tested grafts were statistically significant. Our findings suggest that of the vascular grafts we studied, the Dacron-silver-triclosan and Omniflow II grafts might prevent biofilm formation by MSSA. Although further studies are needed, these grafts seem to be good candidates for clinical use in vascular surgeries at high risk of infections due to this microorganism.IMPORTANCE The Dacron silver-triclosan and Omniflow II vascular grafts showed the greatest resistance to in vitro methicillin-susceptible Staphylococcus aureus biofilm formation compared to other vascular grafts. These findings could allow us to choose the most resistant to infection prosthetic graft. The Dacron silver-triclosan and Omniflow II vascular grafts showed the greatest resistance to in vitro methicillin-susceptible Staphylococcus aureus biofilm formation compared to other vascular grafts.

引用

页数：7