The Study of Antioxidant Activity of Silver Nanoparticles by the Method of Luminol-Activated Chemiluminescence in the H2O2-Horseradish Peroxidase System

The work presents the results of the synthesis and characterization of arabinogalactan-stabilized Ag0NPs nanoparticles, and also the assessment of their radical-binding ability by the luminol-activated chemiluminescence method using the radical-generating horseradish peroxidase-H2O2 system. The obtained water-soluble nanocomposites containing 0.5-5.0% of the inorganic phase are formed as nanoparticles of zero-valent silver stabilized by arabinogalactan macromolecules. The shape of the nanoparticles is close to spherical, and the average size varies in the range of 3.0-5.4 nm. Using the method of activated chemiluminescence and the protocol for processing the obtained TAR values, it was shown for the first time that aqueous solutions of the resulting nanocomposites have antiradical activity with a pronounced concentration-dependent nature.