Involvement of Nrf2 in the immune regulation of Litopenaeus vannamei against Vibrio harveyi infection

被引:11
作者
Yuan, Yunhao [1 ,2 ]
Guan, Haoxiang [1 ,2 ]
Huang, Yongxiong [1 ,2 ]
Luo, Junliang [1 ,2 ]
Jian, Jichang [1 ,2 ]
Cai, Shuanghu [1 ,2 ]
Yang, Shiping [1 ,2 ]
机构
[1] Guangdong Ocean Univ, Guangdong Prov Key Lab Aquat Anim Dis Control & Hl, Fisheries Coll, Zhanjiang, Peoples R China
[2] Guangdong Higher Educ Inst, Key Lab Control Dis Aquat Anim, Zhanjiang 524088, Peoples R China
关键词
Nrf2; Litopenaeus vannamei; Antioxidant capacity; Vibrio harveyi; Inflammation; OXIDATIVE STRESS-RESPONSE; TRANSCRIPTION FACTOR NRF2; GENE; ACTIVATION; INFLAMMATION; PATHWAY; INJURY; MICE; TRANSGLUTAMINASE; MACROPHAGES;
D O I
10.1016/j.fsi.2023.108547
中图分类号
S9 [水产、渔业];
学科分类号
0908 ;
摘要
NF-E2-related factor-like-2 (Nrf2) is a transcription factor that belongs to the Cap'n'Collar transcription factor family and plays a role in regulating inflammation, autophagy, metabolism, proteostasis, and cancer prevention. However, its influence on Vibrio spp infection in L. vannamei remains uncertain. In this study, the effects of Nrf2 on the immune response in Vibrio spp infection was determined by RT-PCR and histopathological analysis. The results showed that RNAi of Nrf2 significantly decreased the expression of antioxidant-related genes (CAT, SOD and GST; p < 0.05), and significantly up-regulated inflammation-related genes (IMD, pro-PO, P38, Toll, Hsp70, NF kappa B and RAB6A; p < 0.05) and the apoptosis gene (caspase3). Under the infection of V. harveyi, histopatho-logical analysis showed that after RNAi of Nrf2, the hepatopancreas of shrimp has an abnormal arrangement of hepatic tubules and vacuolization of hepatocyte; The basement membrane is peeled off and the epithelial cells are massively necrotic. Compared with the RNAi of Nrf2 group, the tissue damage in the SFN group was much lessened, and there were fewer apoptosis signals in the TUNEL assay. In conclusion, this experiment indicated that Nrf2 is involved in the regulation of inflammatory response, oxidative stress,and apoptosis induced by V. harveyi in L. vannamei.
引用
收藏
页数:10
相关论文
共 51 条
[31]   Essential function of transglutaminase and clotting protein in shrimp immunity [J].
Maningas, Mary Beth Bacano ;
Kondo, Hidehiro ;
Hirono, Ikuo ;
Saito-Taki, Tatsuo ;
Aoki, Takashi .
MOLECULAR IMMUNOLOGY, 2008, 45 (05) :1269-1275
[32]   Optimal dietary curcumin improved growth performance, and modulated innate immunity, antioxidant capacity and related genes expression of NF--κB and Nrf2 signaling pathways in grass carp (Ctenopharyngodon idella) after infection with Aeromonas hydrophila [J].
Ming, Jianhua ;
Ye, Jinyun ;
Zhang, Yixiang ;
Xu, Qiyou ;
Yang, Xia ;
Shao, Xianping ;
Qiang, Jun ;
Xu, Pao .
FISH & SHELLFISH IMMUNOLOGY, 2020, 97 :540-553
[33]   Nrf2 Redirects Glucose and Glutamine into Anabolic Pathways in Metabolic Reprogramming [J].
Mitsuishi, Yoichiro ;
Taguchi, Keiko ;
Kawatani, Yukie ;
Shibata, Tatsuhiro ;
Nukiwa, Toshihiro ;
Aburatani, Hiroyuki ;
Yamamoto, Masayuki ;
Motohashi, Hozumi .
CANCER CELL, 2012, 22 (01) :66-79
[34]   Nrf2 regulates the sensitivity of death receptor signals by affecting intracellular glutathione levels [J].
Morito, N ;
Yoh, K ;
Itoh, K ;
Hirayama, A ;
Koyama, A ;
Yamamoto, M ;
Takahashi, S .
ONCOGENE, 2003, 22 (58) :9275-9281
[35]   Helicobacter pylori infection promotes autophagy through Nrf2-mediated heme oxygenase upregulation in human gastric cancer cells [J].
Paik, Ji Yeon ;
Lee, Hee Geum ;
Piao, Juan-Yu ;
Kim, Su-Jung ;
Kim, Do-Hee ;
Na, Hye-Kyung ;
Surh, Young-Joon .
BIOCHEMICAL PHARMACOLOGY, 2019, 162 :89-97
[36]   Effects of Vibro harveyi and Staphyloccocus aureus infection on hemocyanin synthesis and innate immune responses in white shrimp Litopenaeus vannamei [J].
Pan, Luqing ;
Zhang, Xin ;
Yang, Liubing ;
Pan, Shanshan .
FISH & SHELLFISH IMMUNOLOGY, 2019, 93 :659-668
[37]   ROS signaling, oxidative stress and Nrf2 in pancreatic beta-cell function [J].
Pi, Jingbo ;
Zhang, Qiang ;
Fu, Jingqi ;
Woods, Courtney G. ;
Hou, Yongyong ;
Corkey, Barbara E. ;
Collins, Sheila ;
Andersen, Melvin E. .
TOXICOLOGY AND APPLIED PHARMACOLOGY, 2010, 244 (01) :77-83
[38]   Disruption of Nrf2 Impairs the Resolution of Hyperoxia-Induced Acute Lung Injury and Inflammation in Mice [J].
Reddy, Narsa M. ;
Kleeberger, Steven R. ;
Kensler, Thomas W. ;
Yamamoto, Masayuki ;
Hassoun, Paul M. ;
Reddy, Sekhar P. .
JOURNAL OF IMMUNOLOGY, 2009, 182 (11) :7264-7271
[39]   An Overview of Nrf2 Signaling Pathway and Its Role in Inflammation [J].
Saha, Sarmistha ;
Buttari, Brigitta ;
Panieri, Emiliano ;
Profumo, Elisabetta ;
Saso, Luciano .
MOLECULES, 2020, 25 (22)
[40]   Amomum tsao-ko fruit extract suppresses lipopolysaccharide-induced inducible nitric oxide synthase by inducing heme oxygenase-1 in macrophages and in septic mice [J].
Shin, Ji-Sun ;
Ryu, Suran ;
Jang, Dae Sik ;
Cho, Young-Wuk ;
Chung, Eun Kyung ;
Lee, Kyung-Tae .
INTERNATIONAL JOURNAL OF EXPERIMENTAL PATHOLOGY, 2015, 96 (06) :395-405