Illustrate the distribution and metabolic regulatory effects of pterostilbene in cerebral ischemia-reperfusion rat brain by mass spectrometry imaging and spatial metabolomics

被引:9
作者
Ban, Weikang [1 ]
Jiang, Xinyi [2 ]
Lv, Lingjuan [1 ]
Jiao, Yue [3 ]
Huang, Jianpeng [2 ]
Yang, Zhihong [1 ]
You, Yuyang [4 ]
机构
[1] Chinese Acad Med Sci & Peking Union Med Coll, Inst Med Plant Dev, Beijing 100193, Peoples R China
[2] Chinese Acad Med Sci & Peking Union Med Coll, Inst Mat Med, Beijing 100050, Peoples R China
[3] China Acad Chinese Med Sci, Expt Res Ctr, Beijing 100700, Peoples R China
[4] Beijing Inst Technol, Sch Automat, Beijing 100081, Peoples R China
基金
北京市自然科学基金;
关键词
Pterostilbene; Cerebral ischemia-reperfusion; Spatial metabolomics; Mass spectrometry imaging; In situ; FLOW-ASSISTED IONIZATION; ARTERY OCCLUSION; INJURY; STROKE; MECHANISMS; BREAKDOWN; SYSTEM; STRESS; GERBIL; LIPIDS;
D O I
10.1016/j.talanta.2023.125060
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Pterostilbene is a promising molecule with superior pharmacological activities and pharmacokinetic characteristics compared to its structural analogue resveratrol, which could be used to treat ischemic stroke. However, its mechanism is still unclear. The cutting-edge air flow-assisted desorption electrospray ionization mass spectrometry imaging (AFADESI-MSI) and spatial metabolomics analysis were applied to investigate the distribution of pterostilbene in ischemic rat brain and the changes of related small molecule metabolic pathways to further explore the potential mechanisms of pterostilbene against cerebral ischemia-reperfusion injury. This research found that pterostilbene could significantly restore cerebral microcirculation blood flow, reduce infarct volume, improve neurological function and ameliorate neuronal damage in ischemic rats. Moreover, pterostilbene was widely and abundantly distributed in ischemic brain tissue, laying a solid foundation for the rescue of ischemic penumbra. Further study revealed that pterostilbene played a therapeutic role in restoring energy supply, rebalancing neurotransmitters, reducing abnormal polyamine accumulation and phospholipid metabolism. These findings offer an opportunity to illustrate novel mechanisms of pterostilbene in the treatment of cerebral ischemia/reperfusion injury resulting from ischemic stroke.
引用
收藏
页数:13
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