Generation of canine induced pluripotent stem cells under feeder-free conditions using Sendai virus vector encoding six canine reprogramming factors

被引：9

作者：

Tsukamoto, Masaya ^{[1
,2
,3
]}

Kimura, Kazuto ^{[1
,2
]}

Yoshida, Takumi ^{[1
,2
]}

Tanaka, Miyuu ^{[4
,5
]}

Kuwamura, Mitsuru ^{[4
,5
]}

Ayabe, Taro ^{[6
]}

Ishihara, Genki ^{[6
]}

Watanabe, Kei ^{[6
]}

Okada, Mika ^{[7
]}

Iijima, Minoru ^{[7
]}

Nakanishi, Mahito ^{[7
]}

Akutsu, Hidenori ^{[3
]}

Sugiura, Kikuya ^{[1
,2
]}

Hatoya, Shingo ^{[1
,2
]}

机构：

[1] Osaka Metropolitan Univ, Grad Sch Vet Sci, Dept Adv Pathobiol, Izumisano, Osaka 5988531, Japan

[2] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Dept Adv Pathobiol, Izumisano, Osaka 5988531, Japan

[3] Natl Ctr Child Hlth & Dev, Ctr Regenerat Med, Tokyo, Tokyo 1578535, Japan

[4] Osaka Metropolitan Univ, Grad Sch Vet Sci, Dept Integrated Struct Biosci, Izumisano, Osaka 5988531, Japan

[5] Osaka Prefecture Univ, Grad Sch Life & Environm Sci, Dept Integrated Struct Biosci, Izumisano, Osaka 5988531, Japan

[6] Anicom Specialty Med Inst, Shinjuku Ku, Tokyo, Japan

[7] TOKIWA Bio, Tsukuba, Ibaraki 3050047, Japan

来源：

STEM CELL REPORTS | 2024年 / 19卷 / 01期

关键词：

INDUCTION; CULTURE; STATE;

D O I：

10.1016/j.stemcr.2023.11.010

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

Although it is in its early stages, canine induced pluripotent stem cells (ciPSCs) hold great potential for innovative translational research in regenerative medicine, developmental biology, drug screening, and disease modeling. However, almost all ciPSCs were generated from fibroblasts, and available canine cell sources for reprogramming are still limited. Furthermore, no report is available to generate ciPSCs under feeder -free conditions because of their low reprogramming efficiency. Here, we reanalyzed canine pluripotency-associated genes and designed canine LIN28A, NANOG, OCT3/4, SOX2, KLF4, and C-MYC encoding Sendai virus vector, called 159cf. and 162cf. We demonstrated that not only canine fibroblasts but also canine urine -derived cells, which can be isolated using a noninvasive and straightforward method, were successfully reprogrammed with or without feeder cells. ciPSCs existed in undifferentiated states, differentiating into the three germ layers in vitro and in vivo. We successfully generated ciPSCs under feeder -free conditions, which can promote studies in veterinary and consequently human regenerative medicines.

引用

页码：141 / 157

页数：17

共 56 条

[1]

amanaka S., 2018, United States Patent, Patent No. [US20180371036A1, 20180371036]

[2] Reprogramming Factor Stoichiometry Influences the Epigenetic State and Biological Properties of Induced Pluripotent Stem Cells [J].