Vitamin D deficiency and the vitamin D receptor (VDR) gene polymorphism rs2228570 (FokI) are associated with an increased susceptibility to hypertension among the Bangladeshi population

被引:2
作者
Nabil, Imrul Kayes [1 ]
Mahmud, Zimam [1 ]
Tamanna, Sonia [1 ]
Rahman, Md Mostafijur [1 ]
Rashid, Gowhar [2 ]
Hossain, Md. Sarowar [3 ]
Asad, Humaira Binte [1 ]
Kamal, Mohammad Amjad [3 ,4 ,5 ,6 ,7 ]
Howlader, Md. Zakir Hossain [1 ]
机构
[1] Univ Dhaka, Dept Biochem & Mol Biol, Dhaka, Bangladesh
[2] Amity Univ Haryana AUH, Amity Med Sch, Dept Med Lab Technol, Gurugram, India
[3] Daffodil Int Univ, Dept Pharm, Birulia, Bangladesh
[4] Sichuan Univ, West China Hosp, Inst Syst Genet, Frontiers Sci Ctr Dis, Chengdu, Peoples R China
[5] King Abdulaziz Univ, King Fahd Med Res Ctr, Jeddah, Saudi Arabia
[6] Enzymoics, Hebersham, NSW, Australia
[7] Novel Global Community Educ Fdn, Hebersham, NSW, Australia
来源
PLOS ONE | 2024年 / 19卷 / 03期
基金
英国医学研究理事会;
关键词
25-HYDROXYVITAMIN D; BLOOD-PRESSURE; RISK; METABOLISM; SERUM;
D O I
10.1371/journal.pone.0297138
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Vitamin D receptor (VDR) gene is implicated in hypertension vulnerability due to its role in regulating the renin-angiotensin system (RAS) and blood pressure. In this case-control study, a carefully selected cohort of 111 hypertensive individuals and 100 healthy controls underwent serum analysis using HPLC to measure 25-hydroxy vitamin D levels. Polymorphic variations in the VDR gene were detected and characterized using the PCR-RFLP method. At first, lower 25-hydroxy vitamin D levels were observed in hypertensive individuals compared to controls (p<0.001). The genotype frequency of the VDR gene TaqI showed no significant difference between cases and controls (p>0.05). Similarly, no significant difference was found in the VDR gene BsmI genotype frequency between hypertensive patients and controls (p>0.05). However, a statistically significant distinction was observed in the VDR gene FokI genotype frequency between cases and controls (p<0.01). The odds ratios for FokI genotypes (CC, CT, TT, and CT+TT) were 1.0, 0.590, 1.566, and 0.963, respectively. Furthermore, serum 25-hydroxy vitamin D levels were significantly higher in control subjects compared to hypertensive patients across all genotypes of VDR (p<0.001). Hypertensive patients, excluding those with the FokI VDR gene CC genotype, exhibited significantly higher systolic blood pressure levels compared to the control group (p<0.05). Similarly, hypertensive subjects displayed elevated diastolic blood pressure levels compared to the control group (p<0.001). Overall, the results suggest the presence of a potential inverse correlation between serum 25-hydroxy vitamin D levels and hypertension. The association analysis conducted indicated that there is no significant association between TaqI and bsmI genotypic variants and the risk of developing hypertension. However, it was observed that VDR gene polymorphisms do have a clear association with hypertension susceptibility, as evidenced by the significantly higher occurrence of FokI genotypic variants in hypertensive patients. Our study therefore introduces the possibility of utilizing 25-hydroxy vitamin D deficiency and VDR gene polymorphisms as a biomarker for hypertension.
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页数:17
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共 39 条
[1]   Vitamin D status and vitamin D receptor gene polymorphisms and susceptibility to type 1 diabetes in Egyptian children [J].
Abd-Allah, Somia H. ;
Pasha, Heba F. ;
Hagrass, Hoda A. ;
Alghobashy, Ashgan A. .
GENE, 2014, 536 (02) :430-434
[2]   Vitamin D receptor gene TaqI and Apal polymorphisms and steroid responsiveness in childhood idiopathic nephrotic syndrome [J].
Al-Eisa, Amal A. ;
Haider, Mohammad Z. .
INTERNATIONAL JOURNAL OF NEPHROLOGY AND RENOVASCULAR DISEASE, 2016, 9 :187-192
[3]   World Medical Association Declaration of Helsinki Ethical Principles for Medical Research Involving Human Subjects [J].
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 2013, 310 (20) :2191-2194
[4]   An inexpensive, simple protocol for DNA isolation from blood for high-throughput genotyping by polymerase chain reaction or restriction endonuclease digestion [J].
Bailes, S. M. ;
Devers, J. J. ;
Kirby, J. D. ;
Rhoads, D. D. .
POULTRY SCIENCE, 2007, 86 (01) :102-106
[5]   25-Hydroxyvitamin D Levels and Hypertension Rates [J].
Bhandari, Simran K. ;
Pashayan, Shahe ;
Liu, In Lu A. ;
Rasgon, Scott A. ;
Kujubu, Dean A. ;
Tom, Thomas Y. ;
Sim, John J. .
JOURNAL OF CLINICAL HYPERTENSION, 2011, 13 (03) :170-177
[6]   Vitamin D Binding Protein: A Historic Overview [J].
Bouillon, Roger ;
Schuit, Frans ;
Antonio, Leen ;
Rastinejad, Fraydoon .
FRONTIERS IN ENDOCRINOLOGY, 2020, 10
[7]   Association of vitamin D deficiency with arterial stiffness in newly diagnosed hypertension [J].
Cakal, Sinem ;
Cakal, Beytullah ;
Karaca, Oguz .
BLOOD PRESSURE MONITORING, 2021, 26 (02) :113-117
[8]   Vitamin D deficiency and essential hypertension [J].
Chen, Songcang ;
Sun, Yingxian ;
Agrawal, Devendra K. .
JOURNAL OF THE AMERICAN SOCIETY OF HYPERTENSION, 2015, 9 (11) :885-901
[9]   VITAMIN D: METABOLISM, MOLECULAR MECHANISM OF ACTION, AND PLEIOTROPIC EFFECTS [J].
Christakos, Sylvia ;
Dhawan, Puneet ;
Verstuyf, Annemieke ;
Verlinden, Lieve ;
Carmeliet, Geert .
PHYSIOLOGICAL REVIEWS, 2016, 96 (01) :365-408
[10]   Vitamin D Status in Relation to Glucose Metabolism and Type 2 Diabetes in Septuagenarians [J].
Dalgard, Christine ;
Petersen, Maria Skaalum ;
Weihe, Pal ;
Grandjean, Philippe .
DIABETES CARE, 2011, 34 (06) :1284-1288