IL-4 shapes microglia-dependent pruning of the cerebellum during postnatal development

Interleukin-4 (IL-4) is a type 2 cytokine with pleiotropic functions in adaptive immunity, allergies, and cognitive processes. Here, we show that low levels of IL-4 in the early postnatal stage delineate a critical period in which microglia extensively prune cerebellar neurons. Elevating the levels of this cytokine via peripheral in-jection, or using a mouse model of allergic asthma, leads to defective pruning, permanent increase in cere-bellar granule cells, and circuit alterations. These animals also show a hyperkinetic and impulsive-like pheno-type, reminiscent of attention-deficit hyperactivity disorder (ADHD). These alterations are blocked in Il4rafl/fl:: Cx3cr1-CreER mice, which are deficient in IL-4 receptor signaling in microglia. These findings demonstrate a previously unknown role for IL-4 during a neuroimmune critical period of cerebellar maturation and provide a first putative mechanism for the comorbidity between allergic disease and ADHD observed in humans.