The Prevalence of Undiagnosed Salmonella enterica Serovar Typhi in Healthy School-Aged Children in Osun State, Nigeria

被引:1
作者
Uwanibe, Jessica N. N. [1 ,2 ]
Kayode, Tolulope A. A. [1 ,2 ]
Oluniyi, Paul E. E. [1 ,2 ]
Akano, Kazeem [1 ,2 ]
Olawoye, Idowu B. B. [1 ,2 ]
Ugwu, Chinedu A. A. [1 ,2 ]
Happi, Christian T. T. [1 ,2 ]
Folarin, Onikepe A. A. [1 ,2 ]
机构
[1] Redeemers Univ, African Ctr Excellence Genom Infect Dis ACEGID, Ede 232103, Osun, Nigeria
[2] Redeemers Univ, Coll Nat Sci, Dept Biol Sci, Oshogbo 232102, Osun, Nigeria
关键词
Salmonella Typhi; typhoid fever; ELISA; lipopolysaccharide; asymptomatic; next-generation sequencing; FEVER; DIAGNOSIS; PCR; BURDEN; IGM;
D O I
10.3390/pathogens12040594
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Typhoid fever remains a significant public health concern due to cases of mis-/overdiagnosis. Asymptomatic carriers play a role in the transmission and persistence of typhoid fever, especially among children, where limited data exist in Nigeria and other endemic countries. We aim to elucidate the burden of typhoid fever among healthy school-aged children using the best surveillance tool(s). In a semi-urban/urban state (Osun), 120 healthy school-aged children under 15 years were enrolled. Whole blood and fecal samples were obtained from consenting children. ELISA targeting the antigen lipopolysaccharide (LPS) and anti-LPS antibodies of Salmonella Typhi, culture, polymerase chain reaction (PCR), and next-generation sequencing (NGS) were used to analyze the samples. At least one of the immunological markers was detected in 65.8% of children, with 40.8%, 37.5%, and 39% of children testing positive for IgM, IgG, and antigen, respectively. Culture, PCR, and NGS assays did not detect the presence of Salmonella Typhi in the isolates. This study demonstrates a high seroprevalence of Salmonella Typhi in these healthy children but no carriage, indicating the inability to sustain transmission. We also demonstrate that using a single technique is insufficient for typhoid fever surveillance in healthy children living in endemic areas.
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页数:12
相关论文
共 40 条
[1]   Operationalising One Health in Nigeria: Reflections From a High-Level Expert Panel Discussion Commemorating the 2020 World Antibiotics Awareness Week [J].
Achi, Chioma R. ;
Ayobami, Olaniyi ;
Mark, Godwin ;
Egwuenu, Abiodun ;
Ogbolu, David ;
Kabir, Junaidu .
FRONTIERS IN PUBLIC HEALTH, 2021, 9
[2]   Estimating typhoid incidence from community-based serosurveys: a multicohort study [J].
Aiemjoy, Kristen ;
Seidman, Jessica C. ;
Saha, Senjuti ;
Munira, Sira Jam ;
Sajib, Mohammad Saiful Islam ;
Al Sium, Syed Muktadir ;
Sarkar, Anik ;
Alam, Nusrat ;
Zahan, Farha Nusrat ;
Kabir, Md Shakiul ;
Tamrakar, Dipesh ;
Vaidya, Krista ;
Shrestha, Rajeev ;
Shakya, Jivan ;
Katuwal, Nishan ;
Shrestha, Sony ;
Yousafzai, Mohammad Tahir ;
Iqbal, Junaid ;
Dehraj, Irum Fatima ;
Ladak, Yasmin ;
Maria, Noshi ;
Adnan, Mehreen ;
Pervaiz, Sadaf ;
Carter, Alice S. ;
Longley, Ashley T. ;
Fraser, Clare ;
Ryan, Edward T. ;
Nodoushani, Ariana ;
Fasano, Alessio ;
Leonard, Maureen M. ;
Kenyon, Victoria ;
Bogoch, Isaac I. ;
Jeon, Hyon Jin ;
Haselbeck, Andrea ;
Park, Se Eun ;
Zellweger, Raphael M. ;
Marks, Florian ;
Owusu-Dabo, Ellis ;
Adu-Sarkodie, Yaw ;
Owusu, Michael ;
Teunis, Peter ;
Luby, Stephen P. ;
Garrett, Denise O. ;
Qamar, Farah Naz ;
Saha, Samir K. ;
Charles, Richelle C. ;
Andrews, Jason R. .
LANCET MICROBE, 2022, 3 (08) :E578-E587
[3]   Typhoid Fever: Tracking the Trend in Nigeria [J].
Akinyemi, Kabiru Olusegun ;
Oyefolu, Akeeb Oriowo Bola ;
Mutiu, Wasiu Bamidele ;
Iwalokun, Bamidele Abiodun ;
Ayeni, Edward Sunday ;
Ajose, Samuel Oluwasegun ;
Obaro, Stephen K. .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2018, 99 (03) :41-47
[4]   The burden of typhoid fever in low- and middle-income countries: A meta-regression approach [J].
Antillon, Marina ;
Warren, Joshua L. ;
Crawford, Forrest W. ;
Weinberger, Daniel M. ;
Kurum, Esra ;
Pak, Gi Deok ;
Marks, Florian ;
Pitzer, Virginia E. .
PLOS NEGLECTED TROPICAL DISEASES, 2017, 11 (02)
[5]   Typhoid and paratyphoid fever [J].
Bhan, MK ;
Bahl, R ;
Bhatnagar, S .
LANCET, 2005, 366 (9487) :749-762
[6]   Trimmomatic: a flexible trimmer for Illumina sequence data [J].
Bolger, Anthony M. ;
Lohse, Marc ;
Usadel, Bjoern .
BIOINFORMATICS, 2014, 30 (15) :2114-2120
[7]  
Crump JA, 2004, B WORLD HEALTH ORGAN, V82, P346
[8]   Serodiagnosis of Acute Typhoid Fever in Nigerian Pediatric Cases by Detection of Serum IgA and IgG against Hemolysin E and Lipopolysaccharide [J].
Davies, D. Huw ;
Jain, Aarti ;
Nakajima, Rie ;
Liang, Li ;
Jasinskis, Algis ;
Supnet, Medalyn ;
Felgner, Philip L. ;
Teng, Andy ;
Pablo, Jozelyn ;
Molina, Douglas M. ;
Obaro, Stephen K. .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2016, 95 (02) :431-439
[9]   Molecular characterization of invasive Enterobacteriaceae from pediatric patients in Central and Northwestern Nigeria [J].
Duru, Carissa ;
Olanipekun, Grace ;
Odili, Vivian ;
Kocmich, Nicholas ;
Rezac, Amy ;
Ajose, Theresa O. ;
Medugu, Nubwa ;
Umoru, Dominic ;
Onuchukwu, Chukwuma ;
Munir, Huda ;
Jibir, Binta Wudil ;
Farouk, Zubaida ;
Gambo, Safiya ;
Hassan-Hanga, Fatimah ;
Olaosebikan, Rasaq ;
Ebruke, Bernard ;
Esimone, Charles ;
Obaro, Stephen .
PLOS ONE, 2020, 15 (10)
[10]   Investigation of a community outbreak of typhoid fever associated with drinking water [J].
Farooqui, Amber ;
Khan, Adnan ;
Kazmi, Shahana U. .
BMC PUBLIC HEALTH, 2009, 9