Simultaneous adsorption and biodegradation of oxytetracycline in wastewater by Mycolicibacterium sp. immobilized on magnetic biochar

Due to the adverse effects of long-term oxytetracycline (OTC) residues in aquatic environments, an effective treatment is urgently needed. Immobilized microbial technology has been widely explored in the treatment of various organic pollutants in aquatic environments with its excellent environmental adaptability. Nevertheless, studies on its application in the removal of antibiotics are relatively scarce and not in sufficient depth. Only a few studies have further investigated the final fate of antibiotics in the immobilized bacteria system. In this study, a novel kind of OTC-degrading bacteria Mycolicibacterium sp. was immobilized on straw biochar and magnetic biochar, respectively. Magnetic biochar was proved to be a more satisfactory immobilization carrier due to its superior property and the advantage of easy recycling. Compared with free bacteria, immobilized bacteria had stronger environmental adaptability under different OTC concentrations, pH, and heavy metal ions. After 5 cycles, immobilized bacteria could still remove 71.8% of OTC, indicating that it had a stable recyclability. Besides, OTC in real swine wastewater was completely removed by immobilized bacteria within 2 days. The results of FTIR showed that bacteria were successfully immobilized on biochar and O-H, N-H, and C-N groups might be involved in the removal of OTC. The fate analysis indicated that OTC was removed by simultaneous adsorption and biodegradation, while biodegradation (92.8%) played a dominant role in the immobilized bacteria system. Meanwhile, the amount of adsorbed OTC (7.20%) was rather small, which could effectively decrease the secondary pollution of OTC. At last, new degradation pathways of OTC were proposed. This study provides an ecofriendly and effective approach to remedy OTC pollution in wastewater.