A novel multimode biosensor for sensitive detection of AFB1 in food based on Mxenes nano enzymes

被引:40
作者
Kong, Yiqian [1 ]
Zhu, Yinghua [2 ]
Song, Juncheng [1 ]
Liu, Qi [1 ]
Song, Lili [3 ]
Fei, Xiaowei [3 ]
Li, Xiangyang [1 ]
机构
[1] Ludong Univ, Sch Food Engn, Yantai 264025, Shandong, Peoples R China
[2] Ludong Univ, Sch Informat & Elect Engn, Yantai 264025, Shandong, Peoples R China
[3] Shandong Jinsheng Grain Oil & Food Co Ltd, Linyi 276629, Shandong, Peoples R China
基金
国家重点研发计划; 中国国家自然科学基金;
关键词
Peanut; Aflatoxin B 1; Fluorescent; Colorimetric; Smartphone; Sensor; Multimode; Testing; AFLATOXIN-B1; NANOSHEETS; B-1;
D O I
10.1016/j.foodchem.2023.136645
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
In this work, Ti3C2 nano-enzymes (Ti3C2 NEs) materials with simulated peroxidase activity and fluorescence quenching properties were prepared. Then Ti3C2 NEs was functionalized using 6-carboxyfluorescein (FAM) labeled Aflatoxin B1 (AFB1) aptamers to construct a novel multimode nano enzyme biosensor for the detection of AFB1 in peanuts. Based on the fluorescence quenching characteristics and the superior simulated peroxidase activity of Ti3C2 NES and the specific binding of the aptamer to AFB1, the sensitive and rapid fluorescence/ colorimetric/smart phone detection of AFB1 have been achieved, with detection limits of 0.09 ng mL-1, 0.61 ng mL-1 and 0.96 ng mL-1, respectively. The analytical method provided can not only detect AFB1 in multiple modes, but also has a wider detection range, lower limit of detection (LOD) and better recovery rate, and can achieve on-site accurate detection of AFB1 content in peanuts, which has great application potential in the field of food quality testing.
引用
收藏
页数:9
相关论文
共 30 条
[1]   Recent Advances in Nanozymes: From Matters to Bioapplications [J].
Ai, Yongjian ;
Hu, Ze-Nan ;
Liang, Xiaoping ;
Sun, Hong-bin ;
Xin, Hongbo ;
Liang, Qionglin .
ADVANCED FUNCTIONAL MATERIALS, 2022, 32 (14)
[2]   A label-free ultrasensitive microfluidic surface Plasmon resonance biosensor for Aflatoxin B1 detection using nanoparticles integrated gold chip [J].
Bhardwaj, Hema ;
Sumana, Gajjala ;
Marquette, Christophe A. .
FOOD CHEMISTRY, 2020, 307
[3]   Automated Paper-Based Femtogram Sensing Device for Competitive Enzyme-Linked Immunosorbent Assay of Aflatoxin B1 Using Submicroliter Samples [J].
Charernchai, Sumamal ;
Chikae, Miyuki ;
Tue Trong Phan ;
Wonsawat, Wanida ;
Hirose, Daisuke ;
Takamura, Yuzuru .
ANALYTICAL CHEMISTRY, 2022, 94 (12) :5099-5105
[4]   Structural Analysis and Intrinsic Enzyme Mimicking Activities of Ligand-Free PtAg Nanoalloys [J].
Chen, Lizhen ;
Klemeyer, Lars ;
Ruan, Mingbo ;
Liu, Xin ;
Werner, Stefan ;
Xu, Weilin ;
Koeppen, Andrea ;
Buecker, Robert ;
Gonzalez, Marta Gallego ;
Koziej, Dorota ;
Parak, Wolfgang J. ;
Chakraborty, Indranath .
SMALL, 2023, 19 (19)
[5]   A stem-loop like aptasensor for sensitive detection of aflatoxin based on graphene oxide/AuNPs nanocomposite platform [J].
Dadmehr, Mehdi ;
Shahi, Sahar Cheraghi ;
Malekkiani, Mitra ;
Korouzhdehi, Behnaz ;
Tavassoli, Abolfazl .
FOOD CHEMISTRY, 2023, 402
[6]   Ratiometric Fluorescent Metal-Organic Framework Biosensor for Ultrasensitive Detection of Acrylamide [J].
Gan, Ziyu ;
Zhang, Wen ;
Arslan, Muhammad ;
Hu, Xuetao ;
Zhang, Xinai ;
Li, Zhihua ;
Shi, Jiyong ;
Zou, Xiaobo .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2022, 70 (32) :10065-10074
[7]   Aflatoxin B1 (AFB1) production by Aspergillus flavus and Aspergillus parasiticus on ground Nyjer seeds: The effect of water activity and temperature [J].
Gizachew, Dawit ;
Chang, Chih-Hsuan ;
Szonyi, Barbara ;
De La Torre, Sandra ;
Ting, Wei-tsyi Evert .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2019, 296 :8-13
[8]   A simple aptamer-based fluorescent aflatoxin B1 sensor using humic acid as quencher [J].
Guo, Manli ;
Hou, Qin ;
Waterhouse, Geoffrey I. N. ;
Hou, Juying ;
Ai, Shiyun ;
Li, Xiangyang .
TALANTA, 2019, 205
[9]   Application of magnetic solid phase extraction for separation and determination of aflatoxins B1 and B2 in cereal products by high performance liquid chromatography-fluorescence detection [J].
Hashemi, Mandi ;
Taherimaslak, Zohreh ;
Rashidi, Somayeh .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2014, 960 :200-208
[10]   Aflatoxin B1 in Rice: Effects of Storage Duration, Grain Type and Size, Production Site, and Season [J].
Hassan, Hussein F. ;
Kordahi, Rita ;
Dimassi, Hani ;
El Khoury, Andre ;
Daou, Rouaa ;
Alwan, Nisreen ;
Merhi, Samar ;
Haddad, Joyce ;
Karam, Layal .
JOURNAL OF FOOD PROTECTION, 2022, 85 (06) :938-944