Cervicovaginal Microbiota Profiles in Precancerous Lesions and Cervical Cancer among Ethiopian Women

被引：20

作者：

Teka, Brhanu ^{[1
,2
]}

Yoshida-Court, Kyoko ^{[3
]}

Firdawoke, Ededia ^{[1
]}

Chanyalew, Zewditu ^{[4
]}

Gizaw, Muluken ^{[2
,5
,6
,7
]}

Addissie, Adamu ^{[2
,5
]}

Mihret, Adane ^{[1
,8
]}

Colbert, Lauren E. E. ^{[3
]}

Napravnik, Tatiana Cisneros ^{[3
]}

El Alam, Molly B. B. ^{[3
]}

Lynn, Erica J. J. ^{[3
]}

Mezzari, Melissa ^{[9
]}

Anuja, Jhingran ^{[3
]}

Kantelhardt, Eva Johanna ^{[2
,6
]}

Kaufmann, Andreas M. M. ^{[10
,11
,12
]}

Klopp, Ann H. H. ^{[3
]}

Abebe, Tamrat ^{[1
,2
]}

机构：

[1] Addis Ababa Univ, Coll Hlth Sci, Sch Med, Dept Microbiol Immunol & Parasitol, POB 9086, Addis Ababa, Ethiopia

[2] Martin Luther Univ Halle Wittenberg, Global Hlth Working Grp, D-06097 Halle, Germany

[3] Univ Texas MD Anderson Canc Ctr Houston, Dept Radiat Oncol, Houston, TX 77030 USA

[4] St Paul Hosp Millennium Med Coll, Dept Pathol, POB 1271, Addis Ababa, Ethiopia

[5] Addis Ababa Univ, Coll Hlth Sci, Sch Publ Hlth, POB 34, Addis Ababa, Ethiopia

[6] Martin Luther Univ Halle Wittenberg, Inst Med Epidemiol Biometr & Informat, D-06120 Halle, Germany

[7] Addis Ababa Univ, NCD Working Grp, POB 34, Addis Ababa, Ethiopia

[8] Armauer Hansen Res Inst, POB 1005, Addis Ababa, Ethiopia

[9] Baylor Coll Med Alkek, Ctr Mol Discovery, Mol Virol & Microbiol, Houston, TX 77030 USA

[10] Charite Univ Med Berlin, Dept Gynecol, D-10117 Berlin, Germany

[11] Free Univ Berlin, D-10117 Berlin, Germany

[12] Humboldt Univ, D-10117 Berlin, Germany

来源：

MICROORGANISMS | 2023年 / 11卷 / 04期

关键词：

Ethiopia; Tikur Anbessa Specialized Hospital; high-risk HPV; cervical intraepithelial neoplasia; cervical microbiota; HUMAN-PAPILLOMAVIRUS INFECTION; INTRAEPITHELIAL NEOPLASIA; VAGINAL MICROBIOME; HPV;

D O I：

10.3390/microorganisms11040833

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Although high-risk human papillomavirus infection is a well-established risk factor for cervical cancer, other co-factors within the local microenvironment may play an important role in the development of cervical cancer. The current study aimed to characterize the cervicovaginal microbiota in women with premalignant dysplasia or invasive cervical cancer compared with that of healthy women. The study comprised 120 Ethiopian women (60 cervical cancer patients who had not received any treatment, 25 patients with premalignant dysplasia, and 35 healthy women). Cervicovaginal specimens were collected using either an Isohelix DNA buccal swab or an Evalyn brush, and ribosomal RNA sequencing was used to characterize the cervicovaginal microbiota. Shannon and Simpson diversity indices were used to evaluate alpha diversity. Beta diversity was examined using principal coordinate analysis of weighted UniFrac distances. Alpha diversity was significantly higher in patients with cervical cancer than in patients with dysplasia and in healthy women (p < 0.01). Beta diversity was also significantly different in cervical cancer patients compared with the other groups (weighted UniFrac Bray-Curtis, p < 0.01). Microbiota composition differed between the dysplasia and cervical cancer groups. Lactobacillus iners was particularly enriched in patients with cancer, and a high relative abundance of Lactobacillus species was identified in the dysplasia and healthy groups, whereas Porphyromonas, Prevotella, Bacteroides, and Anaerococcus species predominated in the cervical cancer group. In summary, we identified differences in cervicovaginal microbiota diversity, composition, and relative abundance between women with cervical cancer, women with dysplasia, and healthy women. Additional studies need to be carried out in Ethiopia and other regions to control for variation in sample collection.

引用

页数：14