Hsa_circ_0013561 promotes epithelial-mesenchymal transition and tumor progression by regulating ANXA2 via miR-23b-3p in ovarian cancer

被引：7

作者：

Lv, Jia ^{[1
]}

Zhang, Yijun ^{[2
]}

Yang, Mengying ^{[3
]}

Qiao, Lianqiao ^{[2
]}

Wang, Huihui ^{[3
]}

Jiang, Huici ^{[1
]}

Fu, Mingxu ^{[1
]}

Qin, Jinlong ^{[1
]}

Xu, Shaohua ^{[2
]}

机构：

[1] Tongji Univ, Shanghai Peoples Hosp 4, Sch Med, Dept Obstet & Gynecol, Shanghai, Peoples R China

[2] Tongji Univ, Shanghai Matern & Infant Hosp 1, Sch Med, Dept Gynecol, Shanghai, Peoples R China

[3] Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Dept Obstet & Gynecol, Shanghai, Peoples R China

来源：

CANCER GENE THERAPY | 2023年 / 31卷 / 1期

关键词：

COMPREHENSIVE DATABASE; CIRCRNA; RNA;

D O I：

10.1038/s41417-023-00686-z

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Our preliminary experiment discovered that hsa_circ_0013561 was aberrantly expressed in OC. However, the underlying mechanism is unclear. The expression of hsa_circ_0013561 in OC cells and tissues was detected by RT-qPCR and fluorescence in situ hybridization. The effects of hsa_circ_0013561 on the proliferation and metastasis of OC were explored by functional experiments such as cell counting kit-8, transwell, and tumor xenograft models. To mechanistically understand the regulatory role of hsa_circ_0013561, bioinformatics analysis, Western blot, luciferase reporter assay, and a series of rescue experiments were applied. We found that the hsa_circ_0013561 expression was elevated in OC cells and tissues, and was correlated with metastasis formation. Downregulation of hsa_circ_0013561 suppressed the proliferation and migration of OC cells both in vitro and in vivo. Regarding the interactions of hsa_circ_0013561, the luciferase reporter assay verified that miR-23b-3p and Annexin A2 (ANXA2) were its downstream targets. MiR-23b-3p inhibition or ANXA2 overexpression reversed OC cell proliferation, migration, and epithelial-mesenchymal transition (EMT) post-hsa_circ_0013561 silencing. Moreover, ANXA2 overexpression also reversed OC cell migration, proliferation, and EMT after miR-23b-3p upregulation. Our data suggest that hsa_circ_0013561 increases the expression of ANXA2 by regulating miR-23b-3p competitively, resulting in EMT and metastasis of OC. Thus, hsa_circ_0013561 may serve as a novel oncogenic biomarker for OC progression.

引用

页码：108 / 118

页数：11