Hsa_circ_0013561 promotes epithelial-mesenchymal transition and tumor progression by regulating ANXA2 via miR-23b-3p in ovarian cancer

被引:7
作者
Lv, Jia [1 ]
Zhang, Yijun [2 ]
Yang, Mengying [3 ]
Qiao, Lianqiao [2 ]
Wang, Huihui [3 ]
Jiang, Huici [1 ]
Fu, Mingxu [1 ]
Qin, Jinlong [1 ]
Xu, Shaohua [2 ]
机构
[1] Tongji Univ, Shanghai Peoples Hosp 4, Sch Med, Dept Obstet & Gynecol, Shanghai, Peoples R China
[2] Tongji Univ, Shanghai Matern & Infant Hosp 1, Sch Med, Dept Gynecol, Shanghai, Peoples R China
[3] Tongji Univ, Shanghai Peoples Hosp 10, Sch Med, Dept Obstet & Gynecol, Shanghai, Peoples R China
关键词
COMPREHENSIVE DATABASE; CIRCRNA; RNA;
D O I
10.1038/s41417-023-00686-z
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Our preliminary experiment discovered that hsa_circ_0013561 was aberrantly expressed in OC. However, the underlying mechanism is unclear. The expression of hsa_circ_0013561 in OC cells and tissues was detected by RT-qPCR and fluorescence in situ hybridization. The effects of hsa_circ_0013561 on the proliferation and metastasis of OC were explored by functional experiments such as cell counting kit-8, transwell, and tumor xenograft models. To mechanistically understand the regulatory role of hsa_circ_0013561, bioinformatics analysis, Western blot, luciferase reporter assay, and a series of rescue experiments were applied. We found that the hsa_circ_0013561 expression was elevated in OC cells and tissues, and was correlated with metastasis formation. Downregulation of hsa_circ_0013561 suppressed the proliferation and migration of OC cells both in vitro and in vivo. Regarding the interactions of hsa_circ_0013561, the luciferase reporter assay verified that miR-23b-3p and Annexin A2 (ANXA2) were its downstream targets. MiR-23b-3p inhibition or ANXA2 overexpression reversed OC cell proliferation, migration, and epithelial-mesenchymal transition (EMT) post-hsa_circ_0013561 silencing. Moreover, ANXA2 overexpression also reversed OC cell migration, proliferation, and EMT after miR-23b-3p upregulation. Our data suggest that hsa_circ_0013561 increases the expression of ANXA2 by regulating miR-23b-3p competitively, resulting in EMT and metastasis of OC. Thus, hsa_circ_0013561 may serve as a novel oncogenic biomarker for OC progression.
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页码:108 / 118
页数:11
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