Time-lapse live-cell imaging of pyroptosis by confocal microscopy

被引：2
|
作者：
Jiang, Shuai [1 ,2 ,3 ]
Qin, Kunpeng [1 ,2 ,3 ]
Sun, Li [1 ,2 ,3 ]
机构：
[1] Chinese Acad Sci, Inst Oceanol, Ctr Ocean Mega Sci, CAS & Shandong Prov Key Lab Expt Marine Biol, Qingdao, Peoples R China
[2] Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
[3] Univ Chinese Acad Sci, Coll Earth & Planetary Sci, Beijing, Peoples R China
来源：
STAR PROTOCOLS | 2023年 / 4卷 / 04期
基金：
中国国家自然科学基金;
关键词：
GASDERMIN D; CLEAVAGE;
D O I：
10.1016/j.xpro.2023.102708
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Pyroptosis is a type of regulated necrosis executed by gasdermin. Osmotic cell swelling and membrane perforation are the key features of pyroptosis. This protocol presents time-lapse imaging of morphological changes during pyroptosis using a confocal microscope. We describe the step-by-step ectopic expression of gasdermin, cell staining with nuclear and membrane probes, and visualization of pyroptosis by time-lapse imaging. This protocol is applicable to monitoring pyroptosis in various situations. For complete details on the use and execution of this protocol, please refer to Qin et al. (2023).1
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收藏
页数：8
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