Micro-vibration results in vitro-derived bovine blastocysts with greater cryotolerance, epigenetic abnormalities, and a massive transcriptional change

被引：1

作者：

dos Santos, Angelica C. ^{[1
]}

Joaquim, Daniel C. ^{[2
]}

Nociti, Ricardo P. ^{[3
]}

Macabelli, Carolina H. ^{[1
]}

Sampaio, Rafael V. ^{[3
,4
]}

Oliveira, Aline S. ^{[2
]}

Pita, Maico O. ^{[5
]}

de Oliveira, Robinson A. M. ^{[5
]}

da Silveira, Juliano C. ^{[3
]}

Meirelles, Flavio V. ^{[3
]}

Watanabe, Osnir Y. ^{[5
]}

Watanabe, Yeda F. ^{[2
]}

Chiaratti, Marcos R. ^{[1
]}

机构：

[1] Univ Fed Sao Carlos, Ctr Ciencias Biol & Saude, Dept Genet & Evolucao, Rodovia Washington Luis S-N,Km 235, BR-13565905 Sao Carlos, SP, Brazil

[2] Vitrogen Biotecnol Reprod Anim, Cravinhos, SP, Brazil

[3] Univ Sao Paulo, Fac Zootecnia & Engn Alimentos, Dept Med Vet, Pirassununga, SP, Brazil

[4] ST Genet, Navasota, TX USA

[5] WTA Watanabe Tecnol Aplicada, Cravinhos, SP, Brazil

来源：

THERIOGENOLOGY | 2023年 / 196卷

基金：

瑞典研究理事会; 巴西圣保罗研究基金会;

关键词：

Cattle; Oocyte; IVP; Blastocyst; Epigenetic; RNAseq; INNER CELL-MASS; EMBRYO DEVELOPMENT; CULTURE-SYSTEM; MOUSE; INCREASES; PREGNANCY; OOCYTES; METABOLISM; MATURATION; PACKAGE;

D O I：

10.1016/j.theriogenology.2022.11.004

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Much effort has been employed to improve the quality of embryos obtained by in vitro production (IVP) given the relevance of this technology to current livestock systems. In this context, dynamic IVP systems have proved beneficial to the embryo once they mimic fluid flows and mechanical forces resulting from the movement of ciliated cells and muscle contraction in the reproductive tract. In the present study, we sought to confirm these initial findings as well as assess potential molecular consequences to the embryo by applying micro-vibration (45 Hz for 5 s once per 60 min) during both oocyte maturation and embryo culture in cattle. As a result, micro-vibration led to lower incidence of apoptosis in blastocysts following vitrification-thawing. Further analyses revealed epigenetic and transcriptional changes in blastocysts derived from the micro-vibration treatment, with a total of 502 differentially expressed genes. Enrich-ment analyses linked differentially expressed genes to 'Oxidative phosphorylation', 'Cytokine-cytokine receptor interaction', and 'Signaling pathways regulating pluripotency of stem cells'. Yet, a meta-analysis indicated that the transcriptional changes induced by micro-vibration were not toward that of in vivo -derived embryos. In conclusion, micro-vibration increases the cryoresistance of bovine embryos, but caution should be taken given the unclear consequences of epigenetic and transcriptional abnormalities induced by the treatment.(c) 2022 Elsevier Inc. All rights reserved.

引用

页码：214 / 226

页数：13